PMID- 20357507
OWN - NLM
STAT- MEDLINE
DCOM- 20100622
LR  - 20110705
IS  - 1423-0151 (Electronic)
IS  - 1011-7571 (Linking)
VI  - 19
IP  - 3
DP  - 2010
TI  - Simultaneous detection and differentiation of Staphylococcus species in blood 
      cultures using fluorescence in situ hybridization.
PG  - 218-21
LID - 10.1159/000285294 [doi]
AB  - OBJECTIVE: To develop a new protocol for the simultaneous detection and 
      differentiation of Staphylococcus aureus and coagulase-negative staphylococci 
      (CoNS) in blood cultures using fluorescence in situ hybridization (FISH), without 
      cultivation and biotyping. MATERIALS AND METHODS: Oligonucleotide probes were 
      used to target the variable regions of the 16S rRNA of S. aureus and CoNS, the 
      probes were labeled with fluorochrome Cy3 (red signal) and fluorescein 
      isothiocyanate (green signal), respectively. It is not possible to design one 
      probe that will hybridize to all CoNS species. Therefore, in this study two 
      differentially labeled probes (STA and SAU) were mixed and used to detect and 
      differentiate S. aureus from CoNS rapidly in a single smear. Samples of 189 
      positive blood cultures with Gram-positive cocci in clusters and 11 Gram-positive 
      cocci in chains or pairs were included. RESULTS: The FISH assay showed 91.8% 
      sensitivity and 100% specificity for S. aureus and 100% sensitivity and 93.3% 
      specificity for CoNS when hybridized with STA and SAU probes. Meanwhile, the 
      assay showed 91.8% sensitivity and 100% specificity for S. aureus and 100% 
      sensitivity and 90% specificity for CoNS, when hybridized with the SAU probe 
      only. CONCLUSION: Our data showed that the FISH technique was suitable for the 
      simultaneous detection and differentiation of Staphylococcus sp. in blood 
      cultures.
CI  - Copyright (c) 2010 S. Karger AG, Basel.
FAU - Wang, Pei
AU  - Wang P
AD  - Department of Laboratory Medicine, First People's Hospital of Jingmen, Jingmen, 
      China. peiwwien@yahoo.com
LA  - eng
PT  - Journal Article
DEP - 20100329
PL  - Switzerland
TA  - Med Princ Pract
JT  - Medical principles and practice : international journal of the Kuwait University, 
      Health Science Centre
JID - 8901334
RN  - 0 (Coagulase)
RN  - 0 (Fluorescent Dyes)
RN  - 0 (RNA, Bacterial)
RN  - 0 (RNA, Ribosomal, 16S)
SB  - IM
CIN - Med Princ Pract. 2011;20(4):390; author reply 391. PMID: 21577005
MH  - Bacteriological Techniques
MH  - Blood Specimen Collection
MH  - Coagulase/classification
MH  - Fluorescent Dyes
MH  - Humans
MH  - In Situ Hybridization, Fluorescence
MH  - RNA, Bacterial/genetics
MH  - RNA, Ribosomal, 16S/genetics
MH  - Sensitivity and Specificity
MH  - Staphylococcus/*classification/genetics
EDAT- 2010/04/02 06:00
MHDA- 2010/06/23 06:00
CRDT- 2010/04/02 06:00
PHST- 2009/04/16 00:00 [received]
PHST- 2009/07/14 00:00 [accepted]
PHST- 2010/04/02 06:00 [entrez]
PHST- 2010/04/02 06:00 [pubmed]
PHST- 2010/06/23 06:00 [medline]
AID - 000285294 [pii]
AID - 10.1159/000285294 [doi]
PST - ppublish
SO  - Med Princ Pract. 2010;19(3):218-21. doi: 10.1159/000285294. Epub 2010 Mar 29.