PMID- 20358056
OWN - NLM
STAT- MEDLINE
DCOM- 20100907
LR  - 20240511
IS  - 1364-5528 (Electronic)
IS  - 0003-2654 (Print)
IS  - 0003-2654 (Linking)
VI  - 135
IP  - 6
DP  - 2010 Jun
TI  - Sensitive electrochemical immunosensor for matrix metalloproteinase-3 based on 
      single-wall carbon nanotubes.
PG  - 1345-50
LID - 10.1039/c0an00028k [doi]
AB  - A novel electrochemical immunosensor for the detection of matrix 
      metalloproteinase-3 (MMP-3), a cancer biomarker protein, based on vertically 
      aligned single-wall carbon nanotube (SWCNT) arrays is presented. Detection was 
      based on a sandwich immunoassay consisting of horseradish peroxidase (14-16 
      labels) conjugated to a secondary antibody and/or a polymer bead loaded with 
      multi-enzyme labels. Performance was optimized by effective minimization of 
      non-specific binding (NSB) events using Bovine serum albumin (BSA), Tween-20 and 
      optimization of the primary antibody and secondary antibody concentrations. 
      Results provided a detection limit of 0.4 ng mL(-1) (7.7 pM) for the 14-16 label 
      sensor protocol and 4 pg mL(-1) (77 fM) using a multiply enzyme labeled polymeric 
      bead amplification strategy in 10 microL of calf serum. This immunosensor based 
      on SWCNT arrays offers great promise for a rapid, simple, cost-effective method 
      for clinical screening of cancer biomarkers for point-of-care diagnosis.
FAU - Munge, Bernard S
AU  - Munge BS
AD  - Salve Regina University, Department of Chemistry, Newport, Rhode Island 
      02840-4192, USA. bernard.munge@salve.edu
FAU - Fisher, Jacqueline
AU  - Fisher J
FAU - Millord, Lines N
AU  - Millord LN
FAU - Krause, Colleen E
AU  - Krause CE
FAU - Dowd, Richard S
AU  - Dowd RS
FAU - Rusling, James F
AU  - Rusling JF
LA  - eng
GR  - P20 RR016457/RR/NCRR NIH HHS/United States
GR  - P20 RR016457-096312/RR/NCRR NIH HHS/United States
GR  - ES013557/ES/NIEHS NIH HHS/United States
GR  - P20RR016457/RR/NCRR NIH HHS/United States
GR  - R01 ES013557/ES/NIEHS NIH HHS/United States
GR  - R01 ES013557-05/ES/NIEHS NIH HHS/United States
PT  - Journal Article
PT  - Research Support, N.I.H., Extramural
DEP - 20100331
PL  - England
TA  - Analyst
JT  - The Analyst
JID - 0372652
RN  - 0 (Antibodies)
RN  - 0 (Enzymes, Immobilized)
RN  - 0 (Nanotubes, Carbon)
RN  - 0 (Polysorbates)
RN  - EC 3.4.24.17 (Matrix Metalloproteinase 3)
SB  - IM
MH  - Antibodies/immunology/metabolism
MH  - Biosensing Techniques/*methods
MH  - Electrochemical Techniques/*methods
MH  - Enzymes, Immobilized
MH  - Immunoenzyme Techniques/*methods
MH  - Matrix Metalloproteinase 3/*analysis
MH  - Nanotubes, Carbon/*chemistry
MH  - Point-of-Care Systems
MH  - Polysorbates/chemistry
PMC - PMC2923849
MID - NIHMS215031
EDAT- 2010/04/02 06:00
MHDA- 2010/09/08 06:00
PMCR- 2011/06/01
CRDT- 2010/04/02 06:00
PHST- 2010/04/02 06:00 [entrez]
PHST- 2010/04/02 06:00 [pubmed]
PHST- 2010/09/08 06:00 [medline]
PHST- 2011/06/01 00:00 [pmc-release]
AID - 10.1039/c0an00028k [doi]
PST - ppublish
SO  - Analyst. 2010 Jun;135(6):1345-50. doi: 10.1039/c0an00028k. Epub 2010 Mar 31.