PMID- 20358320
OWN - NLM
STAT- MEDLINE
DCOM- 20101122
LR  - 20240209
IS  - 1466-1268 (Electronic)
IS  - 1355-8145 (Print)
IS  - 1355-8145 (Linking)
VI  - 15
IP  - 5
DP  - 2010 Sep
TI  - Identification of potential HLA class I and class II epitope precursors 
      associated with heat shock protein 70 (HSPA).
PG  - 729-41
LID - 10.1007/s12192-010-0184-z [doi]
AB  - Heat shock protein 70 (HSPA) is a molecular chaperone which has been suggested to 
      shuttle human leukocyte antigen (HLA) epitope precursors from the proteasome to 
      the transporter associated with antigen processing. Despite the reported 
      observations that peptides chaperoned by HSPA are an effective source of antigens 
      for cross-priming, little is known about the peptides involved in the process. In 
      this study, we investigated the possible involvement of HSPA in HLA class I or 
      class II antigen presentation and analysed the antigenic potential of the 
      associated peptides. HSPA was purified from CCRF-CEM and K562 cell lines, and 
      using mass spectrometry techniques, we identified 44 different peptides which 
      were co-purified with HSPA. The affinity of the identified peptides to two HSPA 
      isoforms, HSPA1A and HSPA8, was confirmed using a peptide array. Four of the 
      HSPA-associated peptides were matched with 13 previously reported HLA epitopes. 
      Of these 13 peptides, nine were HLA class I and four were HLA class II epitopes. 
      These results demonstrate the association of HSPA with HLA class I and class II 
      epitopes, therefore providing further evidence for the involvement of HSPA in the 
      antigen presentation process.
FAU - Stocki, Pawel
AU  - Stocki P
AD  - Haematological Sciences, Institute of Cellular Medicine, Medical School, 
      Framlington Place, Newcastle University, Newcastle upon Tyne NE2 4HH, UK.
FAU - Morris, Nicholas J
AU  - Morris NJ
FAU - Preisinger, Christian
AU  - Preisinger C
FAU - Wang, Xiao N
AU  - Wang XN
FAU - Kolch, Walter
AU  - Kolch W
FAU - Multhoff, Gabriele
AU  - Multhoff G
FAU - Dickinson, Anne M
AU  - Dickinson AM
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20100401
PL  - Netherlands
TA  - Cell Stress Chaperones
JT  - Cell stress & chaperones
JID - 9610925
RN  - 0 (Epitopes)
RN  - 0 (HSP70 Heat-Shock Proteins)
RN  - 0 (Histocompatibility Antigens Class I)
RN  - 0 (Histocompatibility Antigens Class II)
SB  - IM
MH  - Blotting, Western
MH  - Cell Line, Tumor
MH  - Chromatography, High Pressure Liquid
MH  - Epitopes/*chemistry/*metabolism
MH  - HSP70 Heat-Shock Proteins/*metabolism
MH  - Histocompatibility Antigens Class I/*chemistry/*metabolism
MH  - Histocompatibility Antigens Class II/*chemistry/*metabolism
MH  - Humans
MH  - Mass Spectrometry
PMC - PMC3006634
EDAT- 2010/04/02 06:00
MHDA- 2010/12/14 06:00
PMCR- 2011/03/01
CRDT- 2010/04/02 06:00
PHST- 2009/10/27 00:00 [received]
PHST- 2010/02/23 00:00 [accepted]
PHST- 2010/02/20 00:00 [revised]
PHST- 2010/04/02 06:00 [entrez]
PHST- 2010/04/02 06:00 [pubmed]
PHST- 2010/12/14 06:00 [medline]
PHST- 2011/03/01 00:00 [pmc-release]
AID - S1355-8145(23)00593-X [pii]
AID - 184 [pii]
AID - 10.1007/s12192-010-0184-z [doi]
PST - ppublish
SO  - Cell Stress Chaperones. 2010 Sep;15(5):729-41. doi: 10.1007/s12192-010-0184-z. 
      Epub 2010 Apr 1.