PMID- 20362231
OWN - NLM
STAT- MEDLINE
DCOM- 20100426
LR  - 20191210
IS  - 1873-4456 (Electronic)
IS  - 0165-4608 (Linking)
VI  - 198
IP  - 2
DP  - 2010 Apr 15
TI  - Cytogenetic investigations of chronic lymphocytic leukemia.
PG  - 155-61
LID - 10.1016/j.cancergencyto.2009.12.014 [doi]
AB  - This study aimed to determine which culture method would yield the highest 
      culture success rate, mitotic index, banding resolution, and abnormality rate in 
      investigation of patients with chronic lymphocytic leukemia (CLL). A range of 
      culture techniques for conventional cytogenetic (CC) analyses was compared: 
      24-hour unstimulated, 72 hours incubation with additional fetal calf serum, 72 
      hours stimulation with interleukin 4, 72 hours stimulation with 
      lipopolysaccharide (LPS), 72 hours stimulation with TPA 
      (12-O-tetradecanoylphorbol 13-acetate), and 72 hours stimulation with 
      CpG-oligonucleotide DSP30 + Interleukin-2 (IL-2). CC abnormality rates were also 
      compared to fluorescence in situ hybridization (FISH) results using probes for 
      CLL (LSI D13S319/13q34/CEP 12: LSI ATM/p53). Forty-five samples from 24 patients 
      (consisting of 11 newly diagnosed and 13 previously diagnosed patients) were 
      included. For CC, a 100.0% culture success rate was achieved (n = 45) by means of 
      an EDTA (ethylenediaminetetraacetic acid) peripheral blood sample with an 
      associated 62.5% CC abnormality rate (n = 24). FISH detected an abnormality rate 
      of 75.0% (n = 24). The combined CC and FISH abnormality rate was 87.5% (n = 24). 
      This study demonstrates that CC that uses TPA and DSP30 + IL-2 on EDTA peripheral 
      blood is effective in the investigation of CLL and may be used as a supplement to 
      FISH studies.
CI  - Copyright 2010 Elsevier Inc. All rights reserved.
FAU - Wren, Catherine
AU  - Wren C
AD  - Pathology Department, Royal Hobart Hospital, Hobart, Tasmania, 7000, Australia. 
      cathy.wren@dhhs.tas.gov.au
FAU - Moriarty, Helen
AU  - Moriarty H
FAU - Marsden, Katherine
AU  - Marsden K
FAU - Tegg, Elizabeth
AU  - Tegg E
LA  - eng
PT  - Evaluation Study
PT  - Journal Article
PL  - United States
TA  - Cancer Genet Cytogenet
JT  - Cancer genetics and cytogenetics
JID - 7909240
RN  - 0 (CpG-ODN DSP30)
RN  - 0 (Interleukin-2)
RN  - 0 (Oligonucleotides)
RN  - NI40JAQ945 (Tetradecanoylphorbol Acetate)
SB  - IM
MH  - Adult
MH  - Aged
MH  - Aged, 80 and over
MH  - Cell Culture Techniques
MH  - Cell Proliferation/drug effects
MH  - *Cytogenetic Analysis/methods
MH  - Female
MH  - Humans
MH  - Interleukin-2/pharmacology
MH  - Karyotyping/methods
MH  - Leukemia, Lymphocytic, Chronic, B-Cell/*diagnosis/*genetics
MH  - Male
MH  - Middle Aged
MH  - Mitotic Index
MH  - Oligonucleotides/pharmacology
MH  - Tetradecanoylphorbol Acetate/pharmacology
MH  - Tumor Cells, Cultured
EDAT- 2010/04/07 06:00
MHDA- 2010/04/27 06:00
CRDT- 2010/04/06 06:00
PHST- 2009/07/30 00:00 [received]
PHST- 2009/12/05 00:00 [revised]
PHST- 2009/12/27 00:00 [accepted]
PHST- 2010/04/06 06:00 [entrez]
PHST- 2010/04/07 06:00 [pubmed]
PHST- 2010/04/27 06:00 [medline]
AID - S0165-4608(09)00730-4 [pii]
AID - 10.1016/j.cancergencyto.2009.12.014 [doi]
PST - ppublish
SO  - Cancer Genet Cytogenet. 2010 Apr 15;198(2):155-61. doi: 
      10.1016/j.cancergencyto.2009.12.014.