PMID- 20362292
OWN - NLM
STAT- MEDLINE
DCOM- 20101206
LR  - 20220321
IS  - 1879-1484 (Electronic)
IS  - 0021-9150 (Print)
IS  - 0021-9150 (Linking)
VI  - 211
IP  - 2
DP  - 2010 Aug
TI  - Total lymphocyte deficiency attenuates AngII-induced atherosclerosis in males but 
      not abdominal aortic aneurysms in apoE deficient mice.
PG  - 399-403
LID - 10.1016/j.atherosclerosis.2010.02.034 [doi]
AB  - OBJECTIVE: T and B lymphocytes are associated with atherosclerosis and aneurysms. 
      Angiotensin II (AngII) infusion into hypercholesterolemic mice results in 
      augmentation of atherosclerosis and abdominal aortic aneurysm (AAA) formation. In 
      this study, we determined whether total lymphocyte deficiency reduced 
      AngII-induced vascular diseases. METHODS AND RESULTS: ApoE deficient (apoE -/-) 
      mice were cross-bred with recombination activating gene-1 (Rag-1) deficient mice 
      that lack mature T and B lymphocytes. Heterozygous littermates (Rag-1 +/-) have 
      normal lymphocytic function and served as controls. Male and female apoE -/- mice 
      that were either Rag-1 +/- or -/- were fed a normal laboratory diet and infused 
      with either saline or AngII (1000ng/kg/min) subcutaneously via osmotic mini pump 
      for 28 days. Total lymphocyte deficiency had no significant effect on body weight 
      and systolic blood pressure prior to and during AngII infusion. However, it was 
      associated with decreased serum cholesterol concentrations. AngII infusion 
      increased atherosclerotic lesion area in Rag-1 +/- mice compared to saline 
      (P=0.017 in males and P=0.004 in females). This effect was significantly blunted 
      in Rag-1 -/- male (P=0.044), but not female mice. AngII-infusion promoted 
      increased width of the abdominal aorta, with a greater effect in males. Despite 
      the reduction in atherosclerosis in males, Rag-1 deficiency had no significant 
      effect on AngII-induced aortic dilation in either gender. CONCLUSION: T and B 
      lymphocyte deficiency attenuates AngII-induced atherosclerosis in males but not 
      AAA formation in apoE -/- mice.
CI  - Copyright 2010 Elsevier Ireland Ltd. All rights reserved.
FAU - Uchida, Haruhito A
AU  - Uchida HA
AD  - Saha Cardiovascular Research Center, University of Kentucky, Lexington, KY 40536, 
      USA.
FAU - Kristo, Fjoralba
AU  - Kristo F
FAU - Rateri, Debra L
AU  - Rateri DL
FAU - Lu, Hong
AU  - Lu H
FAU - Charnigo, Richard
AU  - Charnigo R
FAU - Cassis, Lisa A
AU  - Cassis LA
FAU - Daugherty, Alan
AU  - Daugherty A
LA  - eng
GR  - P01 HL080100/HL/NHLBI NIH HHS/United States
GR  - P01 HL080100-02/HL/NHLBI NIH HHS/United States
GR  - P01 HL80100/HL/NHLBI NIH HHS/United States
PT  - Journal Article
PT  - Research Support, N.I.H., Extramural
PT  - Research Support, Non-U.S. Gov't
DEP - 20100304
PL  - Ireland
TA  - Atherosclerosis
JT  - Atherosclerosis
JID - 0242543
RN  - 0 (Apolipoproteins E)
RN  - 11128-99-7 (Angiotensin II)
SB  - IM
MH  - Aneurysm/genetics/pathology
MH  - Angiotensin II/*genetics
MH  - Animals
MH  - Aortic Aneurysm, Abdominal/*genetics
MH  - Apolipoproteins E/*genetics
MH  - Atherosclerosis/*genetics
MH  - B-Lymphocytes/cytology
MH  - Female
MH  - *Gene Expression Regulation
MH  - Genotype
MH  - Hypercholesterolemia/genetics
MH  - Lymphocytes/*cytology
MH  - Male
MH  - Mice
MH  - T-Lymphocytes/cytology
PMC - PMC2900415
MID - NIHMS185431
EDAT- 2010/04/07 06:00
MHDA- 2010/12/14 06:00
PMCR- 2011/08/01
CRDT- 2010/04/06 06:00
PHST- 2009/06/04 00:00 [received]
PHST- 2010/02/19 00:00 [revised]
PHST- 2010/02/24 00:00 [accepted]
PHST- 2010/04/06 06:00 [entrez]
PHST- 2010/04/07 06:00 [pubmed]
PHST- 2010/12/14 06:00 [medline]
PHST- 2011/08/01 00:00 [pmc-release]
AID - S0021-9150(10)00175-9 [pii]
AID - 10.1016/j.atherosclerosis.2010.02.034 [doi]
PST - ppublish
SO  - Atherosclerosis. 2010 Aug;211(2):399-403. doi: 
      10.1016/j.atherosclerosis.2010.02.034. Epub 2010 Mar 4.