PMID- 2036963
OWN - NLM
STAT- MEDLINE
DCOM- 19910702
LR  - 20131121
IS  - 0013-7227 (Print)
IS  - 0013-7227 (Linking)
VI  - 128
IP  - 6
DP  - 1991 Jun
TI  - Tumor necrosis factor-alpha stimulates prolactin release from anterior pituitary 
      cells: a possible involvement of intracellular calcium mobilization.
PG  - 2785-90
AB  - We investigated the effects of tumor necrosis factor-alpha (TNF alpha), a 
      cytokine produced as one aspect of inflammatory reactions, on the intracellular 
      free calcium concentration in single pituitary cells using a calcium-sensitive 
      fluorescent dye, indo-1, and a digital imaging fluorescence microscopic system. 
      TNF alpha induced an increase in intracellular free calcium immediately after 
      administration, reaching a peak after 30 sec and then returning to nearly the 
      basal level after 120 sec in the pituitary cells. The cells responding to TNF 
      alpha constitute about 15% of the pituitary cell population, and these cells 
      never responded to the hypothalamic releasing hormones TRH, GnRH, GH-releasing 
      hormone, or CRH. To define the role of calcium in TNF alpha-induced PRL release, 
      dispersed pituitary cells were exposed to agents that modify TNF alpha-induced 
      calcium mobilization. Such calcium channel blockers as cobalt and verapamil 
      decreased basal and TNF alpha-induced PRL release. A low calcium medium also 
      decreased TNF alpha-induced PRL release. These data suggest that intracellular 
      calcium mobilization may be involved in the process of TNF alpha-induced PRL 
      release.
FAU - Koike, K
AU  - Koike K
AD  - Department of Obstetrics and Gynecology, Osaka University Medical School, Japan.
FAU - Masumoto, N
AU  - Masumoto N
FAU - Kasahara, K
AU  - Kasahara K
FAU - Yamaguchi, M
AU  - Yamaguchi M
FAU - Tasaka, K
AU  - Tasaka K
FAU - Hirota, K
AU  - Hirota K
FAU - Miyake, A
AU  - Miyake A
FAU - Tanizawa, O
AU  - Tanizawa O
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - United States
TA  - Endocrinology
JT  - Endocrinology
JID - 0375040
RN  - 0 (Tumor Necrosis Factor-alpha)
RN  - 9002-62-4 (Prolactin)
RN  - SY7Q814VUP (Calcium)
SB  - IM
MH  - Animals
MH  - Biological Transport
MH  - Calcium/*metabolism/physiology
MH  - Female
MH  - Intracellular Membranes/*metabolism
MH  - Osmolar Concentration
MH  - Pituitary Gland, Anterior/cytology/*metabolism
MH  - Prolactin/*metabolism
MH  - Rats
MH  - Tumor Necrosis Factor-alpha/*pharmacology
EDAT- 1991/06/01 00:00
MHDA- 1991/06/01 00:01
CRDT- 1991/06/01 00:00
PHST- 1991/06/01 00:00 [pubmed]
PHST- 1991/06/01 00:01 [medline]
PHST- 1991/06/01 00:00 [entrez]
AID - 10.1210/endo-128-6-2785 [doi]
PST - ppublish
SO  - Endocrinology. 1991 Jun;128(6):2785-90. doi: 10.1210/endo-128-6-2785.