PMID- 20370574
OWN - NLM
STAT- MEDLINE
DCOM- 20110301
LR  - 20101111
IS  - 1607-842X (Electronic)
IS  - 0891-6934 (Linking)
VI  - 43
IP  - 8
DP  - 2010 Dec
TI  - CD4+ T-cell anergy induced by lin- CD117(c-kit)+ stem cell-derived immature 
      dendritic cells loaded with nuclear antigen derived from Trypanosoma equiperdum.
PG  - 664-71
LID - 10.3109/08916931003674691 [doi]
AB  - Dendritic cells (DCs) are professional antigen-presenting cells, which have the 
      extraordinary capacity to initiate naive T-cell-mediated primary immune 
      responses. To investigate the role of DCs in the induction of antigen-specific 
      tolerance, the immature DCs (imDCs) and mature DCs (mDCs) were generated in vitro 
      from lin(-)CD117(c-kit)(+) stem cells isolated from mice bone marrow. Flow 
      cytometry and confocal microscopy were used to characterize the phenotypes of 
      DCs. These cells were loaded with nuclear antigen derived from Trypanosoma 
      equiperdum and then co-cultured with naive CD4(+) T cells. It was found that 
      imDC-treated T cells had lower proliferation level and cytokine expression of 
      interleukin (IL)-2, IL-4, IL-12, and interferon-gamma compared with mDC-treated T 
      cells. These results demonstrated that the maturation status of DCs is critical 
      for preventing the production of autoantibodies.
FAU - Xia, Yumin
AU  - Xia Y
AD  - Department of Dermatology, Renmin Hospital of Wuhan University, Wuhan, 430060, PR 
      China. ymxia@whu.edu.cn
FAU - Zhang, Yi
AU  - Zhang Y
FAU - Jiang, Shan
AU  - Jiang S
FAU - Cheng, Hong
AU  - Cheng H
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20100407
PL  - England
TA  - Autoimmunity
JT  - Autoimmunity
JID - 8900070
RN  - 0 (Antigens, Nuclear)
RN  - 0 (Cytokines)
RN  - EC 2.7.10.1 (Proto-Oncogene Proteins c-kit)
SB  - IM
MH  - Animals
MH  - Antigens, Nuclear/*immunology
MH  - CD4-Positive T-Lymphocytes/*immunology
MH  - Clonal Anergy/*immunology
MH  - Cytokines/analysis
MH  - Dendritic Cells/*immunology
MH  - Flow Cytometry
MH  - Immunophenotyping
MH  - Male
MH  - Mice
MH  - Mice, Inbred C57BL
MH  - Microscopy, Confocal
MH  - Proto-Oncogene Proteins c-kit/*immunology
MH  - Rats
MH  - Rats, Wistar
MH  - Specific Pathogen-Free Organisms
MH  - Trypanosoma/*immunology
EDAT- 2010/04/08 06:00
MHDA- 2011/03/02 06:00
CRDT- 2010/04/08 06:00
PHST- 2010/04/08 06:00 [entrez]
PHST- 2010/04/08 06:00 [pubmed]
PHST- 2011/03/02 06:00 [medline]
AID - 10.3109/08916931003674691 [doi]
PST - ppublish
SO  - Autoimmunity. 2010 Dec;43(8):664-71. doi: 10.3109/08916931003674691. Epub 2010 
      Apr 7.