PMID- 20371977
OWN - NLM
STAT- MEDLINE
DCOM- 20100706
LR  - 20211203
IS  - 1880-3989 (Electronic)
IS  - 0388-1350 (Linking)
VI  - 35
IP  - 2
DP  - 2010 Apr
TI  - Involvement of autophagy via mammalian target of rapamycin (mTOR) inhibition in 
      tributyltin-induced neuronal cell death.
PG  - 245-51
AB  - Tributyltin chloride (TBT) is a neurotoxic environmental pollutant that inhibits 
      mitochondrial adenosine triphosphate (ATP) synthase. Autophagy is one of the 
      major protein degradation systems induced by a decrease of intracellular ATP 
      following activation of AMP-activated protein kinase (AMPK). Because we 
      previously found that TBT induces activation of AMPK, here we examined whether 
      TBT induces autophagic neuronal death. Exposure of cortical neurons to 500 nM TBT 
      reduced the phosphorylation of mammalian target of rapamycin (mTOR), a regulator 
      of autophagy. An autophagy inhibitor, 3-methyladenine (3-MA), markedly decreased 
      TBT-induced neuronal death. TBT also induced the formation of LC3-II, an 
      autophagy marker. These results suggest that TBT-induced neuronal death is at 
      least partly autophagic.
FAU - Nakatsu, Yusuke
AU  - Nakatsu Y
FAU - Kotake, Yaichiro
AU  - Kotake Y
FAU - Takai, Naoko
AU  - Takai N
FAU - Ohta, Shigeru
AU  - Ohta S
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - Japan
TA  - J Toxicol Sci
JT  - The Journal of toxicological sciences
JID - 7805798
RN  - 0 (Intracellular Signaling Peptides and Proteins)
RN  - 0 (LC3 protein, rat)
RN  - 0 (Microtubule-Associated Proteins)
RN  - 0 (Trialkyltin Compounds)
RN  - 4XDX163P3D (tributyltin)
RN  - 5142-23-4 (3-methyladenine)
RN  - EC 2.7.1.1 (mTOR protein, rat)
RN  - EC 2.7.11.1 (Protein Serine-Threonine Kinases)
RN  - EC 2.7.11.1 (TOR Serine-Threonine Kinases)
RN  - EC 2.7.11.31 (AMP-Activated Protein Kinases)
RN  - JAC85A2161 (Adenine)
SB  - IM
MH  - AMP-Activated Protein Kinases/physiology
MH  - Adenine/analogs & derivatives/pharmacology
MH  - Animals
MH  - Autophagy/*physiology
MH  - Cell Death/drug effects
MH  - Cells, Cultured
MH  - Female
MH  - Intracellular Signaling Peptides and Proteins/*antagonists & inhibitors
MH  - Microtubule-Associated Proteins/metabolism
MH  - Neurons/*drug effects
MH  - Protein Serine-Threonine Kinases/*antagonists & inhibitors
MH  - Rats
MH  - Rats, Wistar
MH  - TOR Serine-Threonine Kinases
MH  - Trialkyltin Compounds/*toxicity
EDAT- 2010/04/08 06:00
MHDA- 2010/07/07 06:00
CRDT- 2010/04/08 06:00
PHST- 2010/04/08 06:00 [entrez]
PHST- 2010/04/08 06:00 [pubmed]
PHST- 2010/07/07 06:00 [medline]
AID - JST.JSTAGE/jts/35.245 [pii]
AID - 10.2131/jts.35.245 [doi]
PST - ppublish
SO  - J Toxicol Sci. 2010 Apr;35(2):245-51. doi: 10.2131/jts.35.245.