PMID- 20373035
OWN - NLM
STAT- MEDLINE
DCOM- 20110321
LR  - 20231213
IS  - 1573-4978 (Electronic)
IS  - 0301-4851 (Linking)
VI  - 38
IP  - 1
DP  - 2011 Jan
TI  - Proteomics-based analysis of novel genes involved in response toward soybean 
      mosaic virus infection.
PG  - 511-21
LID - 10.1007/s11033-010-0135-x [doi]
AB  - Soybean mosaic virus (SMV) is one of the most serious virus diseases of soybean. 
      However, little is known about the molecular basis of the soybean defense 
      mechanism against this pathogen. We identified differentially expressed proteins 
      in soybean leaves infected with SMV by proteomic approaches. Twenty-eight protein 
      spots that showed >/=2-fold difference in intensity were identified between 
      mock-inoculated and SMV-infected samples. Among them, 16 spots were upregulated 
      and 12 spots were downregulated in the SMV-infected samples. We recovered 25 of 
      the 28 differentially expressed proteins from two-dimensional electrophoresis 
      (2-DE) gels. These spots were identified as 16 different proteins by Matrix 
      assisted laser desorption/ionization time-of-flight (MALDI-TOF) mass spectrometry 
      (MS) and tandem TOF/TOF MS, and were potentially involved in protein degradation, 
      defense signal transfer, reactive oxygen, cell wall reinforcement, and energy and 
      metabolism regulation. Gene expression analysis of 13 genes by quantitative real 
      time polymerase chain reaction (qRT-PCR) showed that metabolism genes and 
      photosynthesis genes were downregulated at all time points. One energy gene was 
      downregulated, whereas another energy gene was upregulated at five of the six 
      time points. The other interesting genes that were altered by SMV infection 
      showed changes in transcription over time. This is the first extensive 
      application of proteomics to the SMV-soybean interaction. These results 
      contribute to a better understanding of the molecular basis of soybean's 
      responses to SMV.
FAU - Yang, Hua
AU  - Yang H
AD  - National Center for Soybean Improvement, National Key Laboratory of Crop Genetics 
      and Germplasm Enhancement, Nanjing Agricultural University, Nanjing, 210095, 
      China.
FAU - Huang, Yanping
AU  - Huang Y
FAU - Zhi, Haijian
AU  - Zhi H
FAU - Yu, Deyue
AU  - Yu D
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20100407
PL  - Netherlands
TA  - Mol Biol Rep
JT  - Molecular biology reports
JID - 0403234
RN  - 0 (Electrolytes)
RN  - 0 (Plant Proteins)
RN  - 0 (RNA, Messenger)
RN  - 0 (RNA, Plant)
SB  - IM
MH  - Amino Acid Sequence
MH  - Electrolytes/metabolism
MH  - Electrophoresis, Gel, Two-Dimensional
MH  - Energy Metabolism/genetics
MH  - Gene Expression Regulation, Plant
MH  - Genes, Plant/*genetics
MH  - Molecular Sequence Data
MH  - Mosaic Viruses/*physiology
MH  - Plant Diseases/*genetics/immunology/*virology
MH  - Plant Leaves/genetics/virology
MH  - Plant Proteins/analysis/chemistry/classification
MH  - Protein Processing, Post-Translational
MH  - Proteomics/*methods
MH  - RNA, Messenger/genetics/metabolism
MH  - RNA, Plant/genetics/metabolism
MH  - Reverse Transcriptase Polymerase Chain Reaction
MH  - Signal Transduction/genetics
MH  - Silver Staining
MH  - Glycine max/*genetics/*virology
MH  - Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization
MH  - Transcription, Genetic
EDAT- 2010/04/08 06:00
MHDA- 2011/03/22 06:00
CRDT- 2010/04/08 06:00
PHST- 2009/11/18 00:00 [received]
PHST- 2010/03/23 00:00 [accepted]
PHST- 2010/04/08 06:00 [entrez]
PHST- 2010/04/08 06:00 [pubmed]
PHST- 2011/03/22 06:00 [medline]
AID - 10.1007/s11033-010-0135-x [doi]
PST - ppublish
SO  - Mol Biol Rep. 2011 Jan;38(1):511-21. doi: 10.1007/s11033-010-0135-x. Epub 2010 
      Apr 7.