PMID- 20375561
OWN - NLM
STAT- MEDLINE
DCOM- 20101004
LR  - 20100618
IS  - 1662-8128 (Electronic)
IS  - 1662-811X (Linking)
VI  - 2
IP  - 4
DP  - 2010
TI  - Cross-talk between human dendritic cell subsets influences expression of RNA 
      sensors and inhibits picornavirus infection.
PG  - 360-70
LID - 10.1159/000300568 [doi]
AB  - Dendritic cells (DCs) are professional antigen-presenting cells that provide a 
      link between innate and adaptive immunity. Multiple DC subsets exist and their 
      activation by microorganisms occurs through binding of conserved pathogen-derived 
      structures to so-called pattern recognition receptors (PRRs). In this study we 
      analyzed the expression of PRRs responding to viral RNA in human monocyte-derived 
      DCs (moDCs) under steady-state or pro-inflammatory conditions. We found that mRNA 
      and protein levels for most PRRs were increased under pro-inflammatory 
      conditions, with the most pronounced increases in the RIG-like helicase (RLH) 
      family. Additionally, freshly isolated human plasmacytoid DCs (pDCs) displayed 
      significantly higher levels of TLR7, RIG-I, MDA5 and PKR as compared to myeloid 
      DCs and moDCs. Finally, we demonstrate for the first time that cross-talk between 
      TLR-matured or virus-stimulated pDCs and moDCs leads to a type I 
      interferon-dependent antiviral state in moDCs. This antiviral state was 
      characterized by enhanced RLH expression and protection against picornavirus 
      infection. These findings might represent a novel mechanism by which pDCs can 
      preserve the function and viability of myeloid DCs that are attracted to a site 
      with ongoing infection, thereby optimizing the antiviral immune response.
CI  - (c) 2010 S. Karger AG, Basel.
FAU - Kramer, Matthijs
AU  - Kramer M
AD  - Department of Tumor Immunology, Nijmegen Centre for Molecular Life Sciences, 
      Radboud University Nijmegen Medical Centre, Nijmegen, The Netherlands.
FAU - Schulte, Barbara M
AU  - Schulte BM
FAU - Eleveld-Trancikova, Dagmar
AU  - Eleveld-Trancikova D
FAU - van Hout-Kuijer, Maaike
AU  - van Hout-Kuijer M
FAU - Toonen, Liza W J
AU  - Toonen LW
FAU - Tel, Jurjen
AU  - Tel J
FAU - de Vries, I Jolanda M
AU  - de Vries IJ
FAU - van Kuppeveld, Frank J M
AU  - van Kuppeveld FJ
FAU - Jansen, Bastiaan J H
AU  - Jansen BJ
FAU - Adema, Gosse J
AU  - Adema GJ
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20100320
PL  - Switzerland
TA  - J Innate Immun
JT  - Journal of innate immunity
JID - 101469471
RN  - 0 (Receptors, Pattern Recognition)
RN  - 63231-63-0 (RNA)
SB  - IM
MH  - *Cell Communication
MH  - Cells, Cultured
MH  - *Dendritic Cells/cytology/immunology/physiology
MH  - Humans
MH  - Monocytes/cytology/immunology
MH  - Picornaviridae/pathogenicity
MH  - Picornaviridae Infections/*immunology
MH  - RNA/genetics/*metabolism
MH  - Receptors, Pattern Recognition/genetics/*metabolism
EDAT- 2010/04/09 06:00
MHDA- 2010/10/05 06:00
CRDT- 2010/04/09 06:00
PHST- 2009/09/03 00:00 [received]
PHST- 2010/01/05 00:00 [accepted]
PHST- 2010/04/09 06:00 [entrez]
PHST- 2010/04/09 06:00 [pubmed]
PHST- 2010/10/05 06:00 [medline]
AID - 000300568 [pii]
AID - 10.1159/000300568 [doi]
PST - ppublish
SO  - J Innate Immun. 2010;2(4):360-70. doi: 10.1159/000300568. Epub 2010 Mar 20.