PMID- 20376798
OWN - NLM
STAT- MEDLINE
DCOM- 20100628
LR  - 20100408
IS  - 1003-9406 (Print)
IS  - 1003-9406 (Linking)
VI  - 27
IP  - 2
DP  - 2010 Apr
TI  - [The different signal patterns of two FISH probes in the FISH detection of 
      Ph-positive leukemia and their clinical significance].
PG  - 166-70
LID - 10.3760/cma.j.issn.1003-9406.2010.02.011 [doi]
AB  - OBJECTIVE: To compare the signal patterns of dual color extra-signal BCR/ABL 
      probe (ES-FISH) and dual color dual fusion BCR/ABL probe (D-FISH) in the 
      fluorescence in situ hybridization (FISH) detection of Ph-positive leukemia, and 
      to explore their diagnostic value. METHODS: ES-FISH probe and D-FISH probe were 
      used, respectively, to detect the BCR/ABL fusion gene in 74 cases with typical 
      t(9;22)(q34;q11) and 37 cases with variant t(9;22)(q34;q11) translocation or 
      complex karyotypic abnormalities containing Ph translocation. RESULTS: The 
      BCR/ABL fusion gene in all cases with typical t(9;22)(q34;q11) could be detected 
      by both FISH probes. D-FISH had a signal pattern of 1O1G2F, while ES-FISH showed 
      a signal pattern of 2O1G1F. ES-FISH enables the minor breakpoint cluster region 
      to be identified in 9 cases (12.2% ) of Ph-positive leukemia, whereas D-FISH 
      could not differentiate the minor breakpoint cluster region from major breakpoint 
      cluster region. D-FISH could distinguish simple ABL gene deletion from 
      simultaneous deletion of the ABL and BCR genes in 8 cases (10.8%) of Ph-positive 
      leukemia patients, but ES-FISH could not. For variant Ph translocation or complex 
      karyotypic abnormalities containing Ph translocation, each FISH probe showed four 
      or six types of signal pattern, most of which were atypical. The exact 
      interpretation was dependent on conventional karyotypic analysis and FISH on 
      metaphases. CONCLUSION: ES-FISH and D-FISH probes displayed different signal 
      patterns in Ph-positive leukemia due to their differences in size and covered 
      regions. ES-FISH and D-FISH probes may be selected as better probe for 
      Ph-positive acute lymphocytic leukemia and Ph-positive chronic myeloid leukemia, 
      respectively. When imatinib was used for treatment, there was no preference 
      between ES-FISH and D-FISH probe, because major breakpoint cluster region, minor 
      breakpoint cluster region and partial sequence deletion of derivative chromosome 
      9, would not affect the prognosis of Ph-positive leukemia. However, considering 
      that ES-FISH probe has a better cost-performance than D-FISH probe does, it is 
      recommended as first choice.
FAU - Jiang, Hui
AU  - Jiang H
AD  - Jiangsu Institute of Hematology, the First Affiliated Hospital of Soochow 
      University, Thrombosis and Hemostasis Key Laboratory of the Ministry of Health, 
      Soochow University, Suzhou, Jiangsu, P.R. China.
FAU - Xue, Yong-quan
AU  - Xue YQ
FAU - Pan, Jin-lan
AU  - Pan JL
FAU - Zhang, Jun
AU  - Zhang J
FAU - Dai, Hai-ping
AU  - Dai HP
FAU - Wu, Ya-fang
AU  - Wu YF
FAU - Wang, Yong
AU  - Wang Y
FAU - Shen, Juan
AU  - Shen J
FAU - Chen, Su-ning
AU  - Chen SN
LA  - chi
PT  - English Abstract
PT  - Journal Article
PL  - China
TA  - Zhonghua Yi Xue Yi Chuan Xue Za Zhi
JT  - Zhonghua yi xue yi chuan xue za zhi = Zhonghua yixue yichuanxue zazhi = Chinese 
      journal of medical genetics
JID - 9425197
RN  - EC 2.7.11.1 (Proto-Oncogene Proteins c-bcr)
SB  - IM
MH  - Case-Control Studies
MH  - Chromosomes, Human, Pair 9/genetics
MH  - Humans
MH  - In Situ Hybridization, Fluorescence/*methods
MH  - Karyotyping
MH  - Leukemia, Myelogenous, Chronic, BCR-ABL Positive/*diagnosis/*genetics
MH  - Proto-Oncogene Proteins c-bcr/genetics
EDAT- 2010/04/09 06:00
MHDA- 2010/06/29 06:00
CRDT- 2010/04/09 06:00
PHST- 2010/04/09 06:00 [entrez]
PHST- 2010/04/09 06:00 [pubmed]
PHST- 2010/06/29 06:00 [medline]
AID - 940627032 [pii]
AID - 10.3760/cma.j.issn.1003-9406.2010.02.011 [doi]
PST - ppublish
SO  - Zhonghua Yi Xue Yi Chuan Xue Za Zhi. 2010 Apr;27(2):166-70. doi: 
      10.3760/cma.j.issn.1003-9406.2010.02.011.