PMID- 20376914
OWN - NLM
STAT- MEDLINE
DCOM- 20100810
LR  - 20190815
IS  - 1873-264X (Electronic)
IS  - 0731-7085 (Linking)
VI  - 52
IP  - 5
DP  - 2010 Sep 5
TI  - Simple and practical derivatization procedure for enhanced detection of 
      carboxylic acids in liquid chromatography-electrospray ionization-tandem mass 
      spectrometry.
PG  - 809-18
AB  - A simple and practical derivatization procedure for increasing the detection 
      responses of carboxylic acids in liquid chromatography-electrospray 
      ionization-tandem mass spectrometry (LC-ESI-MS/MS) has been developed. 
      2-Hydrazinopyridine (HP) and 2-picolylamine (PA) rapidly reacted with 
      biologically and clinically important carboxylic acids [chenodeoxycholic acid, 
      glycochenodeoxycholic acid, prostaglandin E2, 
      2-(-carboxyethyl)-6-hydroxy-2,7,8-trimethylchroman (gamma-CEHC),alpha-lipoic 
      acid, homovanillic acid (HVA) and 5-hydroxyindole-3-acetic acid] in the presence 
      of 2,2'-dipyridyl disulfide and triphenylphosphine. The resulting HP- and 
      PA-derivatives were highly responsive in ESI-MS operating in the positive-ion 
      mode and gave characteristic product ions during MS/MS, which enabled the 
      sensitive detection using selected reaction monitoring. Among the two reagents, 
      PA was of more practical use; the detection responses of the PA-derivatives were 
      increased by 9-158-fold over the intact carboxylic acids and the limits of 
      detection were in the low femtomole range (1.5-5.6 fmol on column). The 
      PA-derivatization was successfully applied to a biological sample analysis; the 
      derivatization followed by LC-ESI-MS/MS enabled the detection of trace amounts of 
      bile acids, gamma-CEHC and HVA in human saliva with a simple pretreatment, small 
      sample volume and short analysis time.
FAU - Higashi, Tatsuya
AU  - Higashi T
AD  - School of Pharmaceutical Sciences and Global COE Program, University of Shizuoka, 
      52-1 Yada, Suruga-ku, Shizuoka 422-8526, Japan. higashi@u-shizuoka-ken.ac.jp
FAU - Ichikawa, Takuya
AU  - Ichikawa T
FAU - Inagaki, Shinsuke
AU  - Inagaki S
FAU - Min, Jun Zhe
AU  - Min JZ
FAU - Fukushima, Takeshi
AU  - Fukushima T
FAU - Toyo'oka, Toshimasa
AU  - Toyo'oka T
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - England
TA  - J Pharm Biomed Anal
JT  - Journal of pharmaceutical and biomedical analysis
JID - 8309336
RN  - 0 (Carboxylic Acids)
SB  - IM
MH  - Carboxylic Acids/*analysis
MH  - Chromatography, Liquid/*methods
MH  - Humans
MH  - Limit of Detection
MH  - Saliva/chemistry
MH  - Spectrometry, Mass, Electrospray Ionization/*methods
MH  - Tandem Mass Spectrometry/*methods
EDAT- 2010/04/09 06:00
MHDA- 2010/08/11 06:00
CRDT- 2010/04/09 06:00
PHST- 2010/04/09 06:00 [entrez]
PHST- 2010/04/09 06:00 [pubmed]
PHST- 2010/08/11 06:00 [medline]
AID - S0731-7085(10)00143-3 [pii]
AID - 10.1016/j.jpba.2010.03.001 [doi]
PST - ppublish
SO  - J Pharm Biomed Anal. 2010 Sep 5;52(5):809-18. doi: 10.1016/j.jpba.2010.03.001.