PMID- 2037791
OWN - NLM
STAT- MEDLINE
DCOM- 19910702
LR  - 20190510
IS  - 0022-1899 (Print)
IS  - 0022-1899 (Linking)
VI  - 163
IP  - 6
DP  - 1991 Jun
TI  - Induction of suppurative arthritis in rabbits by Haemophilus endotoxin, tumor 
      necrosis factor-alpha, and interleukin-1 beta.
PG  - 1267-72
AB  - Because Haemophilus influenzae type b (Hib) is the principal cause of suppurative 
      arthritis in young children and its lipooligosaccharide (LOS) is thought to be 
      the main virulence factor, Hib endotoxin was evaluated for its ability to induce 
      synovial inflammation in rabbits. Also, the role of the cytokines tumor necrosis 
      factor-alpha (TNF alpha) and interleukin-1 beta (IL-1 beta) in mediating the 
      synovial inflammatory process was studied. Intraarticular inoculation of 2 pg to 
      20 ng of Hib LOS produced a dose-dependent increase in concentrations of 
      leukocytes and protein in synovial lavage fluid that was significantly modulated 
      by concomitant administration of rabbit TNF alpha- and rabbit IL-1 beta-specific 
      antibodies. Inoculation of joints with either 10(4) IU of rabbit TNF alpha or 10 
      ng recombinant rabbit IL-beta induced synovial inflammatory changes similar to 
      those observed after LOS intraarticular challenge. These data provide evidence 
      for the role of Hib LOS in inducing suppurative arthritis and for the critical 
      participation of TNF alpha and IL-1 beta in the initial events of the synovial 
      inflammatory response.
FAU - Saez-Llorens, X
AU  - Saez-Llorens X
AD  - Department of Pediatrics, University of Texas Southwestern Medical Center, Dallas 
      75235-9063.
FAU - Jafari, H S
AU  - Jafari HS
FAU - Olsen, K D
AU  - Olsen KD
FAU - Nariuchi, H
AU  - Nariuchi H
FAU - Hansen, E J
AU  - Hansen EJ
FAU - McCracken, G H Jr
AU  - McCracken GH Jr
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - United States
TA  - J Infect Dis
JT  - The Journal of infectious diseases
JID - 0413675
RN  - 0 (Endotoxins)
RN  - 0 (Interleukin-1)
RN  - 0 (Tumor Necrosis Factor-alpha)
SB  - IM
MH  - Animals
MH  - Arthritis, Infectious/*etiology
MH  - Endotoxins/*toxicity
MH  - Haemophilus Infections/*etiology
MH  - Haemophilus influenzae
MH  - Interleukin-1/analysis/*physiology
MH  - Leukocyte Count
MH  - Male
MH  - Rabbits
MH  - Synovial Fluid/chemistry/cytology
MH  - Tumor Necrosis Factor-alpha/analysis/*physiology
EDAT- 1991/06/01 00:00
MHDA- 2001/03/28 10:01
CRDT- 1991/06/01 00:00
PHST- 1991/06/01 00:00 [pubmed]
PHST- 2001/03/28 10:01 [medline]
PHST- 1991/06/01 00:00 [entrez]
AID - 10.1093/infdis/163.6.1267 [doi]
PST - ppublish
SO  - J Infect Dis. 1991 Jun;163(6):1267-72. doi: 10.1093/infdis/163.6.1267.