PMID- 20379460
OWN - NLM
STAT- MEDLINE
DCOM- 20100715
LR  - 20211020
IS  - 2092-9382 (Electronic)
IS  - 1011-8942 (Print)
IS  - 1011-8942 (Linking)
VI  - 24
IP  - 2
DP  - 2010 Apr
TI  - Protective effect of catechin on apoptosis of the lens epithelium in rats with 
      N-methyl-N-nitrosourea-induced cataracts.
PG  - 101-7
LID - 10.3341/kjo.2010.24.2.101 [doi]
AB  - PURPOSE: To investigate the effect of catechin on apoptotic cell death in the 
      lens epithelium of rats with cataract. METHODS: Cataract was induced by 
      intraperitoneal injection of 100 mg/kg N-methyl-N-nitrosourea (MNU) to ten 
      day-old Sprague-Dawley rats. The neonatal rats were randomly divided into five 
      groups (n=15 in each group): a control group, and four cataract-induction groups, 
      treated with either 0, 50, 100, 200 mg/kg catechin. We performed slit-lamp 
      biomicroscopic analysis, terminal deoxynucleotidyl transferase-mediated dUTP nick 
      end labeling (TUNEL) assay, Western-blot for Bcl-2 and Bax, and 
      immunohistochemistry for caspase-3. RESULTS: Apoptotic cell death in lens 
      epithelial cells that increased following cataract formation in rats was 
      suppressed by cathechin. CONCLUSIONS: Catechin inhibited cataract-induced 
      apoptotic cell death in the lens epithelium and may prove useful for the 
      prevention of cataract progression.
FAU - Lee, Sung Min
AU  - Lee SM
AD  - Department of Ophthalmology, Myongji Hospital, Kwandong University College of 
      Medicine, Goyang, Korea.
FAU - Ko, Il-Gyu
AU  - Ko IG
FAU - Kim, Sung-Eun
AU  - Kim SE
FAU - Kim, Dong Hee
AU  - Kim DH
FAU - Kang, Byung Nam
AU  - Kang BN
LA  - eng
PT  - Journal Article
DEP - 20100406
PL  - Korea (South)
TA  - Korean J Ophthalmol
JT  - Korean journal of ophthalmology : KJO
JID - 8804513
RN  - 8R1V1STN48 (Catechin)
RN  - EC 3.4.22.- (Caspase 3)
SB  - IM
MH  - Analysis of Variance
MH  - Animals
MH  - Animals, Newborn
MH  - Apoptosis/*drug effects
MH  - Blotting, Western
MH  - Caspase 3/metabolism
MH  - Cataract/chemically induced/*drug therapy
MH  - Catechin/*pharmacology
MH  - Immunoenzyme Techniques
MH  - In Situ Nick-End Labeling
MH  - Lens, Crystalline/*drug effects
MH  - Random Allocation
MH  - Rats
MH  - Rats, Sprague-Dawley
PMC - PMC2850997
OTO - NOTNLM
OT  - Apoptosis
OT  - Cataract
OT  - Catechin
OT  - Crystalline lens
OT  - Epithelial cells
OT  - Rats
COIS- No potential conflict of interest relevant to this article was reported.
EDAT- 2010/04/10 06:00
MHDA- 2010/07/16 06:00
PMCR- 2010/04/01
CRDT- 2010/04/10 06:00
PHST- 2009/06/24 00:00 [received]
PHST- 2010/03/08 00:00 [accepted]
PHST- 2010/04/10 06:00 [entrez]
PHST- 2010/04/10 06:00 [pubmed]
PHST- 2010/07/16 06:00 [medline]
PHST- 2010/04/01 00:00 [pmc-release]
AID - 10.3341/kjo.2010.24.2.101 [doi]
PST - ppublish
SO  - Korean J Ophthalmol. 2010 Apr;24(2):101-7. doi: 10.3341/kjo.2010.24.2.101. Epub 
      2010 Apr 6.