PMID- 20379780
OWN - NLM
STAT- MEDLINE
DCOM- 20100913
LR  - 20211020
IS  - 1573-9686 (Electronic)
IS  - 0090-6964 (Print)
IS  - 0090-6964 (Linking)
VI  - 38
IP  - 6
DP  - 2010 Jun
TI  - Osteochondral interface tissue engineering using macroscopic gradients of 
      bioactive signals.
PG  - 2167-82
LID - 10.1007/s10439-010-0028-0 [doi]
AB  - Continuous gradients exist at osteochondral interfaces, which may be engineered 
      by applying spatially patterned gradients of biological cues. In the present 
      study, a protein-loaded microsphere-based scaffold fabrication strategy was 
      applied to achieve spatially and temporally controlled delivery of bioactive 
      signals in three-dimensional (3D) tissue engineering scaffolds. Bone 
      morphogenetic protein-2 and transforming growth factor-beta(1)-loaded 
      poly(D,L-lactic-co-glycolic acid) microspheres were utilized with a gradient 
      scaffold fabrication technology to produce microsphere-based scaffolds containing 
      opposing gradients of these signals. Constructs were then seeded with human bone 
      marrow stromal cells (hBMSCs) or human umbilical cord mesenchymal stromal cells 
      (hUCMSCs), and osteochondral tissue regeneration was assessed in gradient 
      scaffolds and compared to multiple control groups. Following a 6-week cell 
      culture, the gradient scaffolds produced regionalized extracellular matrix, and 
      outperformed the blank control scaffolds in cell number, glycosaminoglycan 
      production, collagen content, alkaline phosphatase activity, and in some 
      instances, gene expression of major osteogenic and chondrogenic markers. These 
      results suggest that engineered signal gradients may be beneficial for 
      osteochondral tissue engineering.
FAU - Dormer, Nathan H
AU  - Dormer NH
AD  - Bioengineering Program, University of Kansas, Lawrence, KS 66045, USA.
FAU - Singh, Milind
AU  - Singh M
FAU - Wang, Limin
AU  - Wang L
FAU - Berkland, Cory J
AU  - Berkland CJ
FAU - Detamore, Michael S
AU  - Detamore MS
LA  - eng
GR  - R21 DE017673/DE/NIDCR NIH HHS/United States
GR  - T32 GM008359/GM/NIGMS NIH HHS/United States
GR  - T32-GM008359/GM/NIGMS NIH HHS/United States
GR  - 1 R21 DE017673-01/DE/NIDCR NIH HHS/United States
PT  - Journal Article
PT  - Research Support, N.I.H., Extramural
PT  - Research Support, Non-U.S. Gov't
DEP - 20100409
PL  - United States
TA  - Ann Biomed Eng
JT  - Annals of biomedical engineering
JID - 0361512
SB  - IM
MH  - Cells, Cultured
MH  - Equipment Design
MH  - Humans
MH  - Male
MH  - Mesenchymal Stem Cells/*cytology/drug effects/*physiology
MH  - Osteogenesis/drug effects/*physiology
MH  - Signal Transduction/drug effects/physiology
MH  - Tissue Engineering/*instrumentation/methods
MH  - *Tissue Scaffolds
MH  - Young Adult
PMC - PMC3773241
MID - NIHMS478423
EDAT- 2010/04/10 06:00
MHDA- 2010/09/14 06:00
PMCR- 2013/09/14
CRDT- 2010/04/10 06:00
PHST- 2009/12/30 00:00 [received]
PHST- 2010/03/26 00:00 [accepted]
PHST- 2010/04/10 06:00 [entrez]
PHST- 2010/04/10 06:00 [pubmed]
PHST- 2010/09/14 06:00 [medline]
PHST- 2013/09/14 00:00 [pmc-release]
AID - 10.1007/s10439-010-0028-0 [doi]
PST - ppublish
SO  - Ann Biomed Eng. 2010 Jun;38(6):2167-82. doi: 10.1007/s10439-010-0028-0. Epub 2010 
      Apr 9.