PMID- 20382145
OWN - NLM
STAT- MEDLINE
DCOM- 20100628
LR  - 20230522
IS  - 1090-2430 (Electronic)
IS  - 0014-4886 (Print)
IS  - 0014-4886 (Linking)
VI  - 224
IP  - 1
DP  - 2010 Jul
TI  - Urocortin 2 increases c-Fos expression in serotonergic neurons projecting to the 
      ventricular/periventricular system.
PG  - 271-81
LID - 10.1016/j.expneurol.2010.04.002 [doi]
AB  - Serotonin plays an important role in the regulation of anxiety states and 
      physiological responses to aversive stimuli. Intracerebroventricular (i.c.v.) 
      injection of the stress- and anxiety-related neuropeptide urocortin 2 (Ucn 2) 
      increases c-Fos expression in serotonergic neurons in the dorsal (DRD) and caudal 
      (DRC) parts of the dorsal raphe nucleus. These regions contain a subset of 
      serotonergic neurons that projects via the dorsal raphe periventricular tract to 
      periventricular structures, including the subfornical organ and ependymal layer, 
      and to the ventricular system. To determine if Ucn 2 activates 
      ventricle/periventricular-projecting serotonergic neurons in the midbrain raphe 
      complex, we made i.c.v. injections of the retrograde tracer Fluoro-Gold into the 
      lateral ventricle, followed 7 days later by i.c.v. injection of Ucn 2. The DRD at 
      -8.18 mm and the DRC at -8.54 mm and -9.16 mm bregma were analyzed using a 
      combined bright field and immunofluorescence technique. Approximately 40% of the 
      ventricle/periventricular-projecting neurons in the subdivisions sampled were 
      serotonergic. Urocortin 2 increased c-Fos expression in 
      ventricle/periventricular-projecting serotonergic neurons in the DRC and in 
      non-ventricle/periventricular-projecting serotonergic neurons in the DRD and DRC. 
      Of the total population of ventricle/periventricular-projecting serotonergic 
      neurons in the DRC at -8.54 and -9.16 mm bregma, 35% expressed c-Fos following 
      Ucn 2 injections. These data are consistent with previous studies showing that 
      i.c.v. injection of Ucn 2 activates subpopulations of serotonergic neurons 
      restricted to the mid-rostrocaudal DRD and DRC and further demonstrate that these 
      include both subsets of serotonergic neurons that do and do not project to the 
      ventricle/periventricular system.
CI  - Published by Elsevier Inc.
FAU - Hale, Matthew W
AU  - Hale MW
AD  - Department of Integrative Physiology and Center for Neuroscience, University of 
      Colorado, Boulder, CO 80309-0354, USA. matthew.hale@colorado.edu
FAU - Stamper, Christopher E
AU  - Stamper CE
FAU - Staub, Daniel R
AU  - Staub DR
FAU - Lowry, Christopher A
AU  - Lowry CA
LA  - eng
GR  - 068558/Z/02/Z/WT_/Wellcome Trust/United Kingdom
GR  - R01 MH086539/MH/NIMH NIH HHS/United States
GR  - WT_/Wellcome Trust/United Kingdom
GR  - R01MH086539/MH/NIMH NIH HHS/United States
GR  - HHMI/Howard Hughes Medical Institute/United States
GR  - R01 MH086539-01/MH/NIMH NIH HHS/United States
PT  - Journal Article
PT  - Research Support, N.I.H., Extramural
PT  - Research Support, Non-U.S. Gov't
DEP - 20100409
PL  - United States
TA  - Exp Neurol
JT  - Experimental neurology
JID - 0370712
RN  - 0 (Neuronal Tract-Tracers)
RN  - 0 (Proto-Oncogene Proteins c-fos)
RN  - 0 (Urocortins)
RN  - 333DO1RDJY (Serotonin)
RN  - EC 1.14.16.4 (Tryptophan Hydroxylase)
SB  - IM
MH  - Analysis of Variance
MH  - Animals
MH  - Catheters, Indwelling
MH  - Cell Count
MH  - Immunohistochemistry
MH  - Male
MH  - Neuronal Tract-Tracers
MH  - Neurons, Afferent/*drug effects/metabolism
MH  - Proto-Oncogene Proteins c-fos/*metabolism
MH  - Raphe Nuclei/metabolism
MH  - Rats
MH  - Rats, Wistar
MH  - Serotonin/*metabolism
MH  - Third Ventricle/*metabolism
MH  - Tryptophan Hydroxylase/metabolism
MH  - Urocortins/*pharmacology
PMC - PMC2885541
MID - NIHMS196494
EDAT- 2010/04/13 06:00
MHDA- 2010/06/29 06:00
PMCR- 2011/07/01
CRDT- 2010/04/13 06:00
PHST- 2010/02/02 00:00 [received]
PHST- 2010/03/25 00:00 [revised]
PHST- 2010/04/01 00:00 [accepted]
PHST- 2010/04/13 06:00 [entrez]
PHST- 2010/04/13 06:00 [pubmed]
PHST- 2010/06/29 06:00 [medline]
PHST- 2011/07/01 00:00 [pmc-release]
AID - S0014-4886(10)00120-2 [pii]
AID - 10.1016/j.expneurol.2010.04.002 [doi]
PST - ppublish
SO  - Exp Neurol. 2010 Jul;224(1):271-81. doi: 10.1016/j.expneurol.2010.04.002. Epub 
      2010 Apr 9.