PMID- 2038316
OWN - NLM
STAT- MEDLINE
DCOM- 19910703
LR  - 20210526
IS  - 0270-7306 (Print)
IS  - 1098-5549 (Electronic)
IS  - 0270-7306 (Linking)
VI  - 11
IP  - 6
DP  - 1991 Jun
TI  - Organization of the murine and human interleukin-7 receptor genes: two mRNAs 
      generated by differential splicing and presence of a type I-interferon-inducible 
      promoter.
PG  - 3052-9
AB  - To better understand the regulation of interleukin-7 receptor (IL-7R) expression, 
      we have pursued a detailed analysis of the structure of the murine and human 
      IL-7R genes. The genes consist of eight exons, the sizes of which are conserved 
      in mouse and human cells, spread out over 24 kbp (murine) and 19 kbp (human). A 
      differential splicing event results in an mRNA encoding a secreted form of the 
      human IL-7R gene. Primer extension and S1 nuclease analysis show a single 
      transcriptional start site for the murine IL-7R gene. The 5'-flanking region of 
      the murine IL-7R gene contains TATA- and CAAT-like sequences. The promoter region 
      also contains a functional interferon regulatory element, to which the 
      interferon-induced nuclear factors IRF-1 and IRF-2 are capable of binding and 
      which is able to confer interferon-inducible expression on a heterologous gene. 
      There are also potential binding sites for the transcription factors AP-1 and 
      AP-2 as well as multiple glucocorticoid response elements. A fusion gene 
      containing 2.5 kb of murine IL-7R 5' regulatory sequence linked to the bacterial 
      chloramphenicol acetyltransferase gene directed expression of chloramphenicol 
      acetyltransferase activity in murine pre-B-cell line 70Z/3 but not in the mouse 
      fibroblast cell line NIH 3T3. Comparison of the murine and human IL-7R 
      exon/intron boundaries with those of other hematopoietin receptor superfamily 
      members whose exon/intron boundaries are also known reveals a conserved 
      evolutionary structure.
FAU - Pleiman, C M
AU  - Pleiman CM
AD  - Department of Molecular Biology, Immunex Corporation, Seattle, Washington 98101.
FAU - Gimpel, S D
AU  - Gimpel SD
FAU - Park, L S
AU  - Park LS
FAU - Harada, H
AU  - Harada H
FAU - Taniguchi, T
AU  - Taniguchi T
FAU - Ziegler, S F
AU  - Ziegler SF
LA  - eng
PT  - Comparative Study
PT  - Journal Article
PL  - United States
TA  - Mol Cell Biol
JT  - Molecular and cellular biology
JID - 8109087
RN  - 0 (Interferon Type I)
RN  - 0 (Interleukin-7)
RN  - 0 (RNA, Messenger)
RN  - 0 (Receptors, Immunologic)
RN  - 0 (Receptors, Interleukin-7)
SB  - IM
MH  - Amino Acid Sequence
MH  - Animals
MH  - Base Sequence
MH  - Chimera
MH  - Cloning, Molecular
MH  - Exons
MH  - Female
MH  - Genomic Library
MH  - Humans
MH  - Interferon Type I/genetics/*pharmacology
MH  - Interleukin-7/metabolism
MH  - Liver/immunology
MH  - Mice
MH  - Molecular Sequence Data
MH  - Placenta/immunology
MH  - Plasmids
MH  - Polymerase Chain Reaction/methods
MH  - Pregnancy
MH  - Promoter Regions, Genetic/*drug effects
MH  - Protein Biosynthesis
MH  - *RNA Splicing
MH  - RNA, Messenger/*genetics
MH  - Receptors, Immunologic/*genetics
MH  - Receptors, Interleukin-7
MH  - Restriction Mapping
MH  - Sequence Homology, Nucleic Acid
MH  - TATA Box
MH  - Transcription, Genetic
PMC - PMC360143
EDAT- 1991/06/01 00:00
MHDA- 1991/06/01 00:01
PMCR- 1991/06/01
CRDT- 1991/06/01 00:00
PHST- 1991/06/01 00:00 [pubmed]
PHST- 1991/06/01 00:01 [medline]
PHST- 1991/06/01 00:00 [entrez]
PHST- 1991/06/01 00:00 [pmc-release]
AID - 10.1128/mcb.11.6.3052-3059.1991 [doi]
PST - ppublish
SO  - Mol Cell Biol. 1991 Jun;11(6):3052-9. doi: 10.1128/mcb.11.6.3052-3059.1991.