PMID- 20383790
OWN - NLM
STAT- MEDLINE
DCOM- 20110124
LR  - 20211020
IS  - 1420-908X (Electronic)
IS  - 1023-3830 (Linking)
VI  - 59
IP  - 10
DP  - 2010 Oct
TI  - Quercetin and kaempferol suppress immunoglobulin E-mediated allergic inflammation 
      in RBL-2H3 and Caco-2 cells.
PG  - 847-54
LID - 10.1007/s00011-010-0196-2 [doi]
AB  - OBJECTIVE: We investigated the inhibitory effects of quercetin and kaempferol 
      treatment on the suppression of immunoglobulin E (IgE)-mediated allergic 
      responses in relation to intestinal epithelium barrier function in RBL-2H3 and 
      Caco-2 cells. METHODS: RBL-2H3 cells as a model of intestinal mucosa mast cells 
      were treated with flavonols followed by IgE-anti-dinitrophenyl sensitization. The 
      extent of degranulation and the release of pro-inflammatory cytokines were 
      measured. Caco-2 cells were stimulated with interleukin (IL)-4 or IgE-allergen 
      with or without flavonol pretreatment and changes in the expression of CD23 mRNA 
      and mitogen-activated protein kinase (MAPK), and chemokine release were 
      determined. RESULTS: Flavonols inhibited the secretion of allergic mediators in 
      RBL-2H3 cells and suppressed the CD23 mRNA expression and p38 MAPK activation in 
      IL-4 stimulated Caco-2 cells. Flavonols also suppressed IgE-OVA induced extra 
      signal-regulated protein kinase (ERK) activation and chemokine release. 
      CONCLUSIONS: Quercetin and kaempferol effectively suppressed the development of 
      IgE-mediated allergic inflammation of intestinal cell models.
FAU - Lee, Eun-Ju
AU  - Lee EJ
AD  - Department of Food and Nutrition, Sookmyung Women's University, Seoul 140-742, 
      Korea.
FAU - Ji, Geun-Eok
AU  - Ji GE
FAU - Sung, Mi-Kyung
AU  - Sung MK
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20100413
PL  - Switzerland
TA  - Inflamm Res
JT  - Inflammation research : official journal of the European Histamine Research 
      Society ... [et al.]
JID - 9508160
RN  - 0 (Antioxidants)
RN  - 0 (Chemokine CCL20)
RN  - 0 (Interleukin-8)
RN  - 0 (Kaempferols)
RN  - 0 (Receptors, IgE)
RN  - 37341-29-0 (Immunoglobulin E)
RN  - 731P2LE49E (kaempferol)
RN  - 9IKM0I5T1E (Quercetin)
RN  - EC 2.7.11.24 (Extracellular Signal-Regulated MAP Kinases)
RN  - EC 2.7.11.24 (p38 Mitogen-Activated Protein Kinases)
SB  - IM
MH  - Animals
MH  - Antioxidants/chemistry/*pharmacology
MH  - *Caco-2 Cells/drug effects/immunology
MH  - Chemokine CCL20/immunology
MH  - Extracellular Signal-Regulated MAP Kinases/immunology
MH  - Food Hypersensitivity/immunology
MH  - Humans
MH  - Hypersensitivity/*immunology
MH  - Immunoglobulin E/*immunology
MH  - Inflammation/*immunology
MH  - Interleukin-8/immunology
MH  - Intestinal Mucosa/cytology
MH  - Kaempferols/chemistry/immunology/*pharmacology
MH  - Molecular Structure
MH  - Quercetin/chemistry/immunology/*pharmacology
MH  - Receptors, IgE/genetics/immunology
MH  - p38 Mitogen-Activated Protein Kinases/immunology
EDAT- 2010/04/13 06:00
MHDA- 2011/01/25 06:00
CRDT- 2010/04/13 06:00
PHST- 2010/01/07 00:00 [received]
PHST- 2010/03/29 00:00 [accepted]
PHST- 2010/03/16 00:00 [revised]
PHST- 2010/04/13 06:00 [entrez]
PHST- 2010/04/13 06:00 [pubmed]
PHST- 2011/01/25 06:00 [medline]
AID - 10.1007/s00011-010-0196-2 [doi]
PST - ppublish
SO  - Inflamm Res. 2010 Oct;59(10):847-54. doi: 10.1007/s00011-010-0196-2. Epub 2010 
      Apr 13.