PMID- 20384732
OWN - NLM
STAT- MEDLINE
DCOM- 20110224
LR  - 20101102
IS  - 1399-0012 (Electronic)
IS  - 0902-0063 (Linking)
VI  - 24
IP  - 5
DP  - 2010 Sep-Oct
TI  - Determination of mismatched donor HLA in kidney transplant recipients with 
      unknown donor HLA phenotypes.
PG  - E178-81
LID - 10.1111/j.1399-0012.2010.01246.x [doi]
AB  - OBJECTIVE: To determine donor human leukocyte antigen (HLA) from renal allograft 
      biopsies in transplant recipients whose donor HLA phenotype is not known. 
      METHODS: Renal allograft biopsies were obtained from seven renal transplant 
      recipients when indicated for allograft dysfunction or proteinuria. DNA was 
      extracted fresh from allograft specimens, and HLA typing was performed with 
      polymerase chain reaction-specific sequence primers (PCR-SSP) and polymerase 
      chain reaction-sequence-specific oligonucleotides (PCR-SSO). RESULTS: HLA typing 
      of the seven renal allograft biopsies was composed of both recipient and donor 
      HLA phenotypes, allowing the determination of the donor HLA and the degree of HLA 
      mismatching. CONCLUSIONS: Deducing mismatched donor HLA antigens in renal 
      allograft recipients enables detection of donor-specific antibodies, and the 
      management of humoral rejection, and enables more appropriate selection of a 
      donor organ should future retransplantation be required.
CI  - (c) 2010 John Wiley & Sons A/S.
FAU - Kwok, Janette
AU  - Kwok J
AD  - Division of Transplantation and Immunogenetics, Department of Pathology and 
      Clinical Biochemistry, Queen Mary Hospital, Hong Kong SAR, China. 
      kwoksy@ha.org.hk
FAU - Chan, Gavin S W
AU  - Chan GS
FAU - Lam, M F
AU  - Lam MF
FAU - Yan, Tony
AU  - Yan T
FAU - Tang, Lydia
AU  - Tang L
FAU - Kwong, K M
AU  - Kwong KM
FAU - Chan, K W
AU  - Chan KW
FAU - Chan, T M
AU  - Chan TM
LA  - eng
PT  - Journal Article
PL  - Denmark
TA  - Clin Transplant
JT  - Clinical transplantation
JID - 8710240
RN  - 0 (HLA Antigens)
RN  - 9007-49-2 (DNA)
SB  - IM
MH  - DNA/*analysis/genetics
MH  - Feasibility Studies
MH  - Graft Rejection/genetics/*immunology
MH  - HLA Antigens/genetics/*immunology
MH  - Histocompatibility Testing
MH  - Humans
MH  - Immunophenotyping
MH  - Kidney Transplantation/*immunology
MH  - Living Donors
MH  - Polymerase Chain Reaction
MH  - Polymorphism, Single-Stranded Conformational
MH  - Transplantation, Homologous
EDAT- 2010/04/14 06:00
MHDA- 2011/02/25 06:00
CRDT- 2010/04/14 06:00
PHST- 2010/04/14 06:00 [entrez]
PHST- 2010/04/14 06:00 [pubmed]
PHST- 2011/02/25 06:00 [medline]
AID - CTR1246 [pii]
AID - 10.1111/j.1399-0012.2010.01246.x [doi]
PST - ppublish
SO  - Clin Transplant. 2010 Sep-Oct;24(5):E178-81. doi: 
      10.1111/j.1399-0012.2010.01246.x.