PMID- 20385070
OWN - NLM
STAT- MEDLINE
DCOM- 20100622
LR  - 20100413
IS  - 0393-974X (Print)
IS  - 0393-974X (Linking)
VI  - 24
IP  - 1
DP  - 2010 Jan-Mar
TI  - Protective effect of a poly-phytocompound on early stage nephropathy secondary to 
      experimentally-induced diabetes.
PG  - 41-9
AB  - Diabetic nephropathy (DN) is a severe and life-threatening complication of 
      long-standing diabetes. As one of the main causes of end-stage renal disease, the 
      prevention and treatment of DN in early stage, and the slowing down of DN 
      progression are of utmost importance and are topics of several ongoing research 
      studies. Nutraceuticals endowed with antioxidant-anti-inflammatory properties may 
      offer an opportunity of integrative treatment for this condition. Male Wistar 
      rats were randomly assigned to two groups. One group of rats (diabetic group) 
      received a single tail-vein injection of STZ compound (50 mg/kg) under light 
      anaesthesia. A protective dose of 0.5 ml of 5 percent dextrose was given 
      intraperitoneally 30 min before the administration of STZ. One diabetic group was 
      fed a normal pellet diet (group A) while group B was fed the diet added with DTS 
      (panax pseudoginseng, eucommia ulmoides), (Kyotsu Jigyo, Tokyo, Japan) in the 
      proportion of 50/25 (percent weight/weight), at the dose of 50 mg/kg/day 
      throughout the experimental period. At the end of 8 weeks, 24-hour urine was 
      collected for the measurement of the albumin concentration: blood samples were 
      collected for serum biochemistry and the rats were sacrificed for kidney 
      measurement of oxidative stress and histomorphological features. Nephrin and 
      Macrophage Chemoattractant Protein-1 (MCP-1) gene expression were also assessed 
      by fluorescence real-time quantitative PCR after RNA extraction and cDNA 
      synthesis. STZ-treated animals showed significantly increased in lipid 
      peroxidation in the kidney and in proteinuria. DTS supplementation did not affect 
      plasma glucose but significantly decreased malonyldialdehyde (MDA) plasma level 
      and the overall redox parameters together with a partial mitigation of 
      proteinuria. Histological analysis showed also that DTS significantly reduced the 
      glomerular volume together with glomerulosclerosis and interstitial fibrosis 
      score (p less than 0.05), the latter two being correlated to proteinuria (p less 
      than 0.05). DTS supplementation also enabled a reduction of diabetes-induced 
      decrease of nephrin mRNA expression and a 67 percent reduction of MCP-1 mRNA 
      up-regulation (p less than 0.01). Taken altogether, these data show that, besides 
      the mandatory control of glycemia, intervention with a nutraceutical with 
      antioxidant and anti-inflammatory properties may have beneficial effects when 
      integrated in the mainstream of the therapeutic regimen.
FAU - Marotta, F
AU  - Marotta F
AD  - WHO-centre for Biotech and Traditional Medicine, University of Milan, Italy. 
      fmarchimede@libero.it
FAU - Harada, M
AU  - Harada M
FAU - Dallah, E D
AU  - Dallah ED
FAU - Yadav, H
AU  - Yadav H
FAU - Solimene, U
AU  - Solimene U
FAU - Di Lembo, S
AU  - Di Lembo S
FAU - Minelli, E
AU  - Minelli E
FAU - Jain, S
AU  - Jain S
FAU - Chui, D H
AU  - Chui DH
LA  - eng
PT  - Journal Article
PL  - Italy
TA  - J Biol Regul Homeost Agents
JT  - Journal of biological regulators and homeostatic agents
JID - 8809253
RN  - 0 (Blood Glucose)
RN  - 0 (Ccl2 protein, rat)
RN  - 0 (Chemokine CCL2)
RN  - 0 (DNA Primers)
RN  - 0 (Membrane Proteins)
RN  - 0 (RNA, Messenger)
RN  - 0 (nephrin)
SB  - IM
MH  - Animals
MH  - Base Sequence
MH  - Blood Glucose/metabolism
MH  - Chemokine CCL2/genetics
MH  - DNA Primers/genetics
MH  - Diabetes Mellitus, Experimental/*drug therapy/genetics/metabolism/pathology
MH  - Diabetic Nephropathies/genetics/metabolism/pathology/*prevention & control
MH  - Female
MH  - Lipid Peroxidation/drug effects
MH  - Male
MH  - Membrane Proteins/genetics
MH  - Oxidative Stress/drug effects
MH  - *Panax
MH  - *Phytotherapy
MH  - RNA, Messenger/genetics/metabolism
MH  - Rats
MH  - Rats, Wistar
EDAT- 2010/04/14 06:00
MHDA- 2010/06/23 06:00
CRDT- 2010/04/14 06:00
PHST- 2010/04/14 06:00 [entrez]
PHST- 2010/04/14 06:00 [pubmed]
PHST- 2010/06/23 06:00 [medline]
AID - 5 [pii]
PST - ppublish
SO  - J Biol Regul Homeost Agents. 2010 Jan-Mar;24(1):41-9.