PMID- 20385283
OWN - NLM
STAT- MEDLINE
DCOM- 20100714
LR  - 20191210
IS  - 1531-5037 (Electronic)
IS  - 0022-3468 (Linking)
VI  - 45
IP  - 4
DP  - 2010 Apr
TI  - Hoxd-13 expression in the development of hindgut in ethylenethiourea-exposed 
      fetal rats.
PG  - 755-61
LID - 10.1016/j.jpedsurg.2009.11.011 [doi]
AB  - PURPOSE: Hoxd-13, as one of the most posterior genes among Hox genes, was 
      reported to play a critical role in the development of the most posterior 
      alimentary canal in vertebrates. This study investigated the expression pattern 
      of Hoxd-13 in the hindgut development of the normal and ethylenethiourea 
      (ETU)-exposed rat embryos with anorectal malformations (ARMs) to find out the 
      possible role of Hoxd-13 in the hindgut development and anorectal morphogenesis. 
      MATERIAL AND METHOD: The ETU murine model of ARMs was used via ETU 1% (125 mg/kg) 
      on gestational day (gD) 10. Embryos were harvested via cesarean delivery on gD13 
      to gD21. Temporal and spatial expression of Hoxd-13 was evaluated in the normal 
      fetal rats (n = 215) and ARMs rats (n = 218) using immunohistochemistry staining, 
      reverse transcriptase polymerase chain reaction, and Western blot analysis. 
      RESULTS: Immunohistochemistry staining revealed that Hoxd-13 expression was 
      confined to the epithelium of the hindgut, cloacal membrane, and urogenital sinus 
      as well as the mesenchyme of the urorectal septum at all gestations in the normal 
      group; however, in the ARMs group, the signal specific for Hoxd-13 was weak in 
      the epithelium of the hindgut and cloacal membrane as well as the mesenchyme of 
      the urorectal septum. Western blot analysis and reverse transcriptase polymerase 
      chain reaction revealed that the level of Hoxd-13 expression was significantly 
      decreased in the ARMs embryos compared with that in the normal embryos on gD13 to 
      gD16 (P < .05) rather than on gD18 to gD21. CONCLUSIONS: The aberrations in 
      spatiotemporal expression pattern of Hoxd-13 on gD13 to gD16 suggested that 
      Hoxd-13 may be an essential inductive signal for normal development of the 
      hindgut, and altered expression may contribute to the abnormal development of the 
      hindgut and accordingly lead to ARMs.
CI  - Copyright 2010 Elsevier Inc. All rights reserved.
FAU - Dan, Zhang
AU  - Dan Z
AD  - Department of Pediatric Surgery, The Second Affiliated Hospital, China Medical 
      University, Shenyang 110004, PR China.
FAU - Bo, Zhang Zhi
AU  - Bo ZZ
FAU - Tao, Zhang
AU  - Tao Z
FAU - Wei, Zhang Shi
AU  - Wei ZS
FAU - Jia, Wang Da
AU  - Jia WD
FAU - Cheng, Zhang Shu
AU  - Cheng ZS
FAU - Wei, Yuan Zheng
AU  - Wei YZ
FAU - Wang, Wei-Lin
AU  - Wang WL
LA  - eng
PT  - Evaluation Study
PT  - Journal Article
PL  - United States
TA  - J Pediatr Surg
JT  - Journal of pediatric surgery
JID - 0052631
RN  - 0 (Homeodomain Proteins)
RN  - 0 (Transcription Factors)
RN  - 24FOJ4N18S (Ethylenethiourea)
SB  - IM
MH  - Anal Canal/*abnormalities/embryology
MH  - Animals
MH  - Blotting, Western
MH  - Digestive System Abnormalities/*embryology/genetics/metabolism
MH  - Ethylenethiourea
MH  - Female
MH  - Homeodomain Proteins/genetics/*metabolism
MH  - Immunohistochemistry
MH  - Male
MH  - Rats
MH  - Rats, Wistar
MH  - Rectum/*abnormalities/embryology
MH  - Reverse Transcriptase Polymerase Chain Reaction
MH  - Transcription Factors/genetics/*metabolism
EDAT- 2010/04/14 06:00
MHDA- 2010/07/16 06:00
CRDT- 2010/04/14 06:00
PHST- 2009/05/07 00:00 [received]
PHST- 2009/11/12 00:00 [revised]
PHST- 2010/11/12 00:00 [accepted]
PHST- 2010/04/14 06:00 [entrez]
PHST- 2010/04/14 06:00 [pubmed]
PHST- 2010/07/16 06:00 [medline]
AID - S0022-3468(09)00942-7 [pii]
AID - 10.1016/j.jpedsurg.2009.11.011 [doi]
PST - ppublish
SO  - J Pediatr Surg. 2010 Apr;45(4):755-61. doi: 10.1016/j.jpedsurg.2009.11.011.