PMID- 20388037
OWN - NLM
STAT- MEDLINE
DCOM- 20101229
LR  - 20211020
IS  - 1937-335X (Electronic)
IS  - 1937-3341 (Print)
IS  - 1937-3341 (Linking)
VI  - 16
IP  - 9
DP  - 2010 Sep
TI  - Umbilical cord stem cell seeding on fast-resorbable calcium phosphate bone 
      cement.
PG  - 2743-53
LID - 10.1089/ten.TEA.2009.0757 [doi]
AB  - Tissue engineering offers immense promise for bone regeneration. Human umbilical 
      cord mesenchymal stem cells (hUCMSCs) can be collected without invasive 
      procedures required for bone marrow MSCs. The objective of this study was to 
      investigate the physical properties and the differentiation capacity of hUCMSCs 
      on calcium phosphate cement (CPC) scaffolds with improved dissolution/resorption 
      rates. CPC consisted of tetracalcium phosphate and dicalcium phosphate anhydrous, 
      with various tetracalcium phosphate/dicalcium phosphate anhydrous ratios. At 1/3 
      ratio, CPC had a dissolution rate 40% faster than CPC control at 1/1. The 
      faster-resorbable CPC had strength and modulus similar to CPC control. Their 
      strength and modulus exceeded the reported values for cancellous bone, and were 
      much higher than those of hydrogels and injectable polymers for cell delivery. 
      hUCMSCs attached to the nano-apatitic CPC and proliferated rapidly. hUCMSCs 
      differentiated into the osteogenic lineage, with significant increases in 
      alkaline phosphatase activity, osteocalcin, collagen I, and osterix gene 
      expression. In conclusion, in this study we reported that hUCMSCs attaching to 
      CPC with high dissolution/resorption rate showed excellent proliferation and 
      osteogenic differentiation. hUCMSCs delivered via high-strength CPC have the 
      potential to be an inexhaustible and low-cost alternative to the gold-standard 
      human bone marrow mesenchymal stem cells. These results may broadly impact 
      stem-cell-based tissue engineering.
FAU - Xu, Hockin H K
AU  - Xu HH
AD  - Department of Endodontics, Prosthodontics, and Operative Dentistry, University of 
      Maryland Dental School, Baltimore, Maryland 21201, USA. hxu@umaryland.edu
FAU - Zhao, Liang
AU  - Zhao L
FAU - Detamore, Michael S
AU  - Detamore MS
FAU - Takagi, Shozo
AU  - Takagi S
FAU - Chow, Laurence C
AU  - Chow LC
LA  - eng
GR  - R01 DE014190/DE/NIDCR NIH HHS/United States
GR  - R01DE11789/DE/NIDCR NIH HHS/United States
GR  - R01DE17974/DE/NIDCR NIH HHS/United States
GR  - R01DE14190/DE/NIDCR NIH HHS/United States
PT  - Journal Article
PT  - Research Support, N.I.H., Extramural
PT  - Research Support, Non-U.S. Gov't
PL  - United States
TA  - Tissue Eng Part A
JT  - Tissue engineering. Part A
JID - 101466659
RN  - 0 (Bone Cements)
RN  - 0 (Calcium Phosphates)
SB  - IM
MH  - Bone Cements/*chemistry/*metabolism
MH  - Calcium Phosphates/*chemistry/*metabolism
MH  - Humans
MH  - Stem Cells/*cytology
MH  - Tissue Engineering/*methods
MH  - Tissue Scaffolds
MH  - Umbilical Cord/*cytology
PMC - PMC2928047
EDAT- 2010/04/15 06:00
MHDA- 2010/12/30 06:00
PMCR- 2011/09/01
CRDT- 2010/04/15 06:00
PHST- 2010/04/15 06:00 [entrez]
PHST- 2010/04/15 06:00 [pubmed]
PHST- 2010/12/30 06:00 [medline]
PHST- 2011/09/01 00:00 [pmc-release]
AID - 10.1089/ten.tea.2009.0757 [pii]
AID - 10.1089/ten.TEA.2009.0757 [doi]
PST - ppublish
SO  - Tissue Eng Part A. 2010 Sep;16(9):2743-53. doi: 10.1089/ten.TEA.2009.0757.