PMID- 20388233
OWN - NLM
STAT- MEDLINE
DCOM- 20100902
LR  - 20100602
IS  - 1469-8161 (Electronic)
IS  - 0031-1820 (Linking)
VI  - 137
IP  - 7
DP  - 2010 Jun
TI  - Der-p2 ( Dermatophagoides pteronyssinus) allergen-like protein from the hard tick 
      Ixodes ricinus - a novel member of ML (MD-2-related lipid-recognition) domain 
      protein family.
PG  - 1139-49
LID - 10.1017/S0031182009992083 [doi]
AB  - OBJECTIVE: Expression of the gene encoding Der-p2 allergen-like protein in the 
      castor bean tick Ixodes ricinus is induced by blood intake. Tick Der-p2 
      allergen-like protein belongs to a diverse family of ML proteins that includes 
      major allergens of house dust mites, human MD-2 or similar proteins from 
      Drosophila melanogaster. In ticks, genes encoding proteins belonging to the ML 
      protein family were identified, but their protein products have not been 
      characterized yet. METHODS: A gene encoding tick Der-p2 allergen-like protein was 
      amplified from cDNA of engorged I. ricinus female using the gene-specific primers 
      designed on a basis of partial sequences of related allergen-like genes. The 
      tissue and state specific patterns of expression of the gene were analysed. The 
      IgE binding activity of the produced recombinant protein was studied by use of 
      ELISA. RESULTS: Analysis of the expression pattern showed that the gene encoding 
      the tick Der-p2 allergen-like protein is strongly induced by the bloodmeal in gut 
      and haemolymph throughout all tick developmental stages. Der-p2 allergen-like 
      protein possesses a putative lipid-binding site, according to the comparisons 
      with the related proteins. The ability of tick Der-p2 allergen-like protein to 
      bind immunoglobulin E (IgE) was revealed. DISCUSSION: The presence of a putative 
      lipid-binding domain in Der-p2 allergen-like protein and its ability to interact 
      with IgE might indicate the involvement of the protein in the tick's immune 
      response.
FAU - Horackova, J
AU  - Horackova J
AD  - Faculty of Science, University of South Bohemia and Biology Centre AS CR, 
      Institute of Parasitology, Branisovska 31, 370 05 Ceske Budejovice, Czech 
      Republic. janah@paru.cas.cz
FAU - Rudenko, N
AU  - Rudenko N
FAU - Golovchenko, M
AU  - Golovchenko M
FAU - Grubhoffer, L
AU  - Grubhoffer L
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20100414
PL  - England
TA  - Parasitology
JT  - Parasitology
JID - 0401121
RN  - 0 (Allergens)
RN  - 0 (Antigens, Dermatophagoides)
RN  - 0 (Lymphocyte Antigen 96)
RN  - 0 (Recombinant Proteins)
RN  - 37341-29-0 (Immunoglobulin E)
SB  - IM
MH  - Allergens/chemistry/genetics/*immunology/metabolism
MH  - Amino Acid Sequence
MH  - Animals
MH  - Antigens, Dermatophagoides/chemistry/genetics/*immunology/metabolism
MH  - Dermatophagoides pteronyssinus/*immunology
MH  - Female
MH  - Immunoglobulin E/immunology/metabolism
MH  - Ixodes/genetics/*growth & development/*immunology/metabolism
MH  - Larva/growth & development/immunology
MH  - Lymphocyte Antigen 96/chemistry/immunology
MH  - Models, Molecular
MH  - Nymph/growth & development/immunology
MH  - Recombinant Proteins/chemistry/genetics/immunology/metabolism
MH  - Sequence Analysis, DNA
EDAT- 2010/04/15 06:00
MHDA- 2010/09/03 06:00
CRDT- 2010/04/15 06:00
PHST- 2010/04/15 06:00 [entrez]
PHST- 2010/04/15 06:00 [pubmed]
PHST- 2010/09/03 06:00 [medline]
AID - S0031182009992083 [pii]
AID - 10.1017/S0031182009992083 [doi]
PST - ppublish
SO  - Parasitology. 2010 Jun;137(7):1139-49. doi: 10.1017/S0031182009992083. Epub 2010 
      Apr 14.