PMID- 20388337
OWN - NLM
STAT- MEDLINE
DCOM- 20100610
LR  - 20131121
IS  - 0253-9624 (Print)
IS  - 0253-9624 (Linking)
VI  - 44
IP  - 2
DP  - 2010 Feb
TI  - [Effects of in utero exposure to di(2-ethylhexyl) phthalate on sexual development 
      in female offspring].
PG  - 150-3
AB  - OBJECTIVE: To evaluate the ability of di(2-ethylhexyl) phthalate (DEHP) with 
      inducing damage in sexual development of female offspring rats after maternal 
      exposure. METHODS: On gestational day (GD) 12, pregnant Wistar rats were weighed, 
      encoded and randomly assigned to 5 groups (10 dams per group). From GD 12 through 
      GD 17 each dam was dosed daily by gavage with either corn oil (vehicle control, 1 
      mgxkg(-1)xd(-1)) or DEHP (1, 250, 750 and 1000 mgxkg(-1)xd(-1)). Then female 
      offspring were monitored for eye opening on postnatal day (PND) 14-17, organ 
      coefficient on PND 22 and the time to vaginal opening on PND 30 - 38 (if vagina 
      did not open during the period, observation time should extent to adult), as well 
      as body weight, time to first estrus. RESULTS: No significant changes were 
      observed on eye opening at any dose, which were (15.8 +/- 0.4) d, (16.3 +/- 0.6) 
      d, (16.0 +/- 0.6) d, (15.9 +/- 0.6) d, (15.8 +/- 0.4) d respectively in control, 
      1, 250, 750 and 1000 mgxkg(-1)xd(-1) (F = 1.363, P = 0.262). However, 62.50% 
      (15/24), 81.25% (26/32) female offspring were permanently absence of vaginal 
      orifice in 750 and 1000 mgxkg(-1)xd(-1) groups respectively, while control, 1 and 
      250 mgxkg(-1)xd(-1) groups developed normally with vaginal orifices (chi(2) 
      values were 84.92, 132.79, respectively, P < 0.01). The ages of vaginal opening 
      were (32.7 +/- 1.3) d, (33.3 +/- 1.5) d, (32.2 +/- 1.5) d, (33.1 +/- 1.3) d, 
      (33.3 +/- 1.2) d and the body weight were (91.56 +/- 6.65) g, (93.79 +/- 6.28) g, 
      (92.98 +/- 8.48) g, (100.57 +/- 6.47) g, (103.83 +/- 8.24) g in control, 1, 250, 
      750 and 1000 mgxkg(-1)xd(-1). After covariance adjustment for body weight, which 
      can statistically influenced the age of vaginal opening (F = 40.857, P < 0.05), 
      difference were found at the age of vaginal opening (F = 3.075, P < 0.05), and 
      250 mgxkg(-1)xd(-1) group was advanced than control (t = -2.056, P < 0.05). 
      CONCLUSION: Exposure to DEHP in utero from GD 12 - 17 can result in abnormalities 
      of sexual development such as the time to vaginal opening and vaginal atresia.
FAU - Ding, Yu
AU  - Ding Y
AD  - Department of Environmental Health, School of Public Health, Shanghai Jiaotong 
      University School of Medicine, Shanghai 200025, China.
FAU - Gao, Yu
AU  - Gao Y
FAU - Shi, Rong
AU  - Shi R
FAU - Zhou, Yi-Jun
AU  - Zhou YJ
FAU - Tian, Ying
AU  - Tian Y
LA  - chi
PT  - English Abstract
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - China
TA  - Zhonghua Yu Fang Yi Xue Za Zhi
JT  - Zhonghua yu fang yi xue za zhi [Chinese journal of preventive medicine]
JID - 7904962
RN  - C42K0PH13C (Diethylhexyl Phthalate)
SB  - IM
MH  - Animals
MH  - Diethylhexyl Phthalate/*toxicity
MH  - Female
MH  - *Maternal Exposure
MH  - Pregnancy
MH  - Rats
MH  - Rats, Wistar
MH  - Sexual Development/*drug effects
MH  - Vagina/*abnormalities
EDAT- 2010/04/15 06:00
MHDA- 2010/06/11 06:00
CRDT- 2010/04/15 06:00
PHST- 2010/04/15 06:00 [entrez]
PHST- 2010/04/15 06:00 [pubmed]
PHST- 2010/06/11 06:00 [medline]
PST - ppublish
SO  - Zhonghua Yu Fang Yi Xue Za Zhi. 2010 Feb;44(2):150-3.