PMID- 20388359
OWN - NLM
STAT- MEDLINE
DCOM- 20100610
LR  - 20201209
IS  - 0253-9624 (Print)
IS  - 0253-9624 (Linking)
VI  - 44
IP  - 1
DP  - 2010 Jan
TI  - [The antagonistic action of epigallocatechin-3-gallate on microcystin LR-induced 
      oxidative damage on hepatocytes of mice and the expression of cytochrome P450 
      2E1].
PG  - 24-9
AB  - OBJECTIVE: To evaluate the effects of antagonistic action of 
      epigallocatechin-3-gallate (EGCG) on microcystin LR (MC-LR) induced oxidative 
      damage on mice and the expression of cytochrome P450 2E1 (CYP2E1) which was one 
      of phase Iota detoxification enzymes. METHODS: A total of 24 specific pathogen 
      free (SPF) male BALB/c mice were randomly divided into four groups, including 
      control group, MC-LR group, low concentration EGCG group, and high concentration 
      EGCG group. Mice were sacrificed on the 15th day, body weight, and the relative 
      organ weight, liver antioxidant enzyme level and lipid peroxidation product, 
      liver histopathology and CYP2E1 gene and protein expression were detected and 
      analyzed respectively. RESULTS: (1) EGCG could antagonise the liver injury which 
      had been damaged by MC-LR. (2) The malonaldehyde (MDA) level ((2.87 +/- 0.03) 
      nmol/mg prot) and superoxide dismutase (SOD) level ((168.18 +/- 2.86) U/mg prot) 
      in MC-LR group were significantly different when compared with the two EGCG 
      treatment groups (the MDA values of the low and high concentration EGCG group 
      were (2.37 +/- 0.05) nmol/mg prot and (1.44 +/- 0.05) nmol/mg prot, F = 906.63, P 
      < 0.01; the SOD values were (176.55 +/- 2.98) U/mg prot and (184.89 +/- 1.53) 
      U/mg prot, F = 32.32, P < 0.01). (3) MC-LR up-regulated the mRNA and protein 
      expression of CYP2E1 (the mRNA values of MC-LR group and control were 1.41 +/- 
      0.26, 0.86 +/- 0.13, t = -4.22, P = 0.003; the protein values of MC-LR group and 
      control were 0.24 +/- 0.03, 0.12 +/- 0.02, t = -9.21, P < 0.05). EGCG 
      down-regulated the mRNA (the values of the low and high concentration EGCG group 
      were 1.09 +/- 0.08, 0.99 +/- 0.09, F = 9.03, P = 0.004) and protein expression 
      (the values of the low and high concentration EGCG group were 0.21 +/- 0.03, 0.14 
      +/- 0.02, F = 24.76, P < 0.05) of CYP2E1 which activated by MC-LR. CONCLUSION: 
      The up-regulation of CYP2E1 which induced by MC-LR was inhibited by EGCG 
      intervention. EGCG might antagonize the oxidation damage of hepatocytes in a 
      certain degree.
FAU - Han, Zhi-Xia
AU  - Han ZX
AD  - Department of Environmental Hygiene, School of Military Preventive Medicine, The 
      Third Military Medical University, Chongqing 400038, China.
FAU - Yang, Lan
AU  - Yang L
FAU - Zhang, Liang
AU  - Zhang L
FAU - Xu, Chuan
AU  - Xu C
FAU - Shu, Wei-Qun
AU  - Shu WQ
LA  - chi
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - China
TA  - Zhonghua Yu Fang Yi Xue Za Zhi
JT  - Zhonghua yu fang yi xue za zhi [Chinese journal of preventive medicine]
JID - 7904962
RN  - 0 (Marine Toxins)
RN  - 0 (Microcystins)
RN  - 8R1V1STN48 (Catechin)
RN  - BQM438CTEL (epigallocatechin gallate)
RN  - EC 1.14.13.- (Cytochrome P-450 CYP2E1)
RN  - EQ8332842Y (cyanoginosin LR)
SB  - IM
MH  - Animals
MH  - Catechin/*analogs & derivatives/pharmacology
MH  - Cytochrome P-450 CYP2E1/*metabolism
MH  - Hepatocytes/*drug effects/*metabolism
MH  - Male
MH  - Marine Toxins
MH  - Mice
MH  - Mice, Inbred BALB C
MH  - Microcystins/adverse effects
MH  - Oxidative Stress/*drug effects
EDAT- 2010/04/15 06:00
MHDA- 2010/06/11 06:00
CRDT- 2010/04/15 06:00
PHST- 2010/04/15 06:00 [entrez]
PHST- 2010/04/15 06:00 [pubmed]
PHST- 2010/06/11 06:00 [medline]
PST - ppublish
SO  - Zhonghua Yu Fang Yi Xue Za Zhi. 2010 Jan;44(1):24-9.