PMID- 20390264
OWN - NLM
STAT- MEDLINE
DCOM- 20110119
LR  - 20211020
IS  - 1432-184X (Electronic)
IS  - 0095-3628 (Linking)
VI  - 60
IP  - 3
DP  - 2010 Oct
TI  - Effects of sponge bleaching on ammonia-oxidizing Archaea: distribution and 
      relative expression of ammonia monooxygenase genes associated with the barrel 
      sponge Xestospongia muta.
PG  - 561-71
LID - 10.1007/s00248-010-9662-1 [doi]
AB  - Sponge-mediated nitrification is an important process in the nitrogen cycle, 
      however, nothing is known about how nitrification and symbiotic Archaea may be 
      affected by sponge disease and bleaching events. The giant barrel sponge 
      Xestospongia muta is a prominent species on Caribbean reefs that contains 
      cyanobacterial symbionts, the loss of which results in two types of bleaching: 
      cyclic, a recoverable condition; and fatal, a condition associated with the 
      disease-like sponge orange band (SOB) syndrome and sponge death. Terminal 
      restriction fragment length polymorphism (TRFLP) analyses, clone libraries, and 
      relative mRNA quantification of ammonia monooxygenase genes (amoA) were performed 
      using a RNA transcript-based approach to characterize the active 
      ammonia-oxidizing Archaea (AOA) community present in bleached, non-bleached, and 
      SOB tissues of cyclically and fatally bleached sponges. We found that 
      non-bleached and cyclically bleached tissues of X. muta harbored a unique 
      Crenarchaeota community closely related to those reported for other sponges. In 
      contrast, bleached tissue from the most degraded sponge contained a Crenarchaeota 
      community that was more similar to those found in sediment and sand. Although 
      there were no significant differences in amoA expression among the different 
      tissues, amoA expression was higher in the most deteriorated tissues. Results 
      suggest that a shift in the Crenarchaeota community precedes an increase in amoA 
      gene expression in fatally bleached sponges, while cyclic bleaching did not alter 
      the AOA community structure and its amoA gene expression.
FAU - Lopez-Legentil, Susanna
AU  - Lopez-Legentil S
AD  - Center for Marine Science, University of North Carolina Wilmington, 5600 Marvin 
      K. Moss Lane, Wilmington, NC 28409, USA. susanna@univ-perp.fr
FAU - Erwin, Patrick M
AU  - Erwin PM
FAU - Pawlik, Joseph R
AU  - Pawlik JR
FAU - Song, Bongkeun
AU  - Song B
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PT  - Research Support, U.S. Gov't, Non-P.H.S.
PL  - United States
TA  - Microb Ecol
JT  - Microbial ecology
JID - 7500663
RN  - 0 (Archaeal Proteins)
RN  - 0 (RNA, Archaeal)
RN  - 7664-41-7 (Ammonia)
RN  - EC 1.- (Oxidoreductases)
RN  - EC 1.7.3.- (ammonia monooxygenase)
SB  - IM
MH  - Ammonia/*metabolism
MH  - Animals
MH  - Archaeal Proteins/genetics
MH  - Crenarchaeota/*enzymology/genetics
MH  - Gene Library
MH  - Genes, Archaeal
MH  - Oxidation-Reduction
MH  - Oxidoreductases/*genetics
MH  - Phylogeny
MH  - Polymorphism, Restriction Fragment Length
MH  - RNA, Archaeal/genetics
MH  - Xestospongia/*microbiology
EDAT- 2010/04/15 06:00
MHDA- 2011/01/20 06:00
CRDT- 2010/04/15 06:00
PHST- 2009/11/06 00:00 [received]
PHST- 2010/03/22 00:00 [accepted]
PHST- 2010/04/15 06:00 [entrez]
PHST- 2010/04/15 06:00 [pubmed]
PHST- 2011/01/20 06:00 [medline]
AID - 10.1007/s00248-010-9662-1 [doi]
PST - ppublish
SO  - Microb Ecol. 2010 Oct;60(3):561-71. doi: 10.1007/s00248-010-9662-1.