PMID- 20390540
OWN - NLM
STAT- MEDLINE
DCOM- 20100706
LR  - 20100414
IS  - 1465-3338 (Electronic)
IS  - 0307-9457 (Linking)
VI  - 39
IP  - 2
DP  - 2010 Apr
TI  - Evaluation of factors influencing replication of serotype 1 Marek's disease 
      vaccines in the chicken lung.
PG  - 71-9
LID - 10.1080/03079451003599250 [doi]
AB  - Factors influencing replication of serotype 1 Marek's disease vaccines in the 
      lung of chickens within the first 10 days of age (doa) were evaluated. In 
      particular, the effect of vaccine efficacy, age/route of vaccination, and vaccine 
      dose were examined in three experiments. In the first experiment, three vaccine 
      pairs, each pair consisting of a high protective (HP) and a low protective (LP) 
      vaccine (CVI988/BP5 and CVI988-Clone C, 648A80 and 648A100, R2 and R2/23) were 
      used to inoculate chickens subcutaneously (s.c.) with 2000 plaque-forming units 
      (PFU) at hatch. DNA load in the lung was significantly higher in the HP vaccine 
      group than the LP vaccine group at 5 and 10 doa in two of the three vaccine 
      pairs. In the lung, at 5 doa, early MDV gene transcripts (ICP4 and pp38) were 
      detected in most vaccine groups, whereas late MDV gene transcripts (gB and gI) 
      were detected only in the HP vaccine group. In the second experiment, chickens 
      were vaccinated in ovo or s.c. at hatch with 2000 PFU R2, R2/23, or CVI988/BP5. 
      Compared with s.c. vaccination, in ovo vaccination resulted in higher MDV DNA 
      load in the lung at 3 doa, lower or equal MDV DNA load at 5 doa, and lower MDV 
      DNA load at 10 doa. In the third experiment, chickens were vaccinated s.c. at 
      hatch with either 2000 or 10,000 PFU strain R2. There were no statistically 
      significant differences in the load of MDV DNA in the lung after vaccination with 
      R2 between the two doses. Our results showed that HP vaccines tend to replicate 
      better than LP vaccines in the lung; and vaccine replication in the lung within 
      the first 10 days of age was affected by the age/route of inoculation (in ovo 
      versus s.c.) but not by the dose of vaccine administered.
FAU - Gimeno, Isabel M
AU  - Gimeno IM
AD  - Department of Population Health and Pathobiology, North Carolina State 
      University, Raleigh, USA. Isabel_Gimeno@ncsu.edu
FAU - Cortes, Aneg L
AU  - Cortes AL
LA  - eng
PT  - Journal Article
PL  - England
TA  - Avian Pathol
JT  - Avian pathology : journal of the W.V.P.A
JID - 8210638
RN  - 0 (DNA, Viral)
RN  - 0 (Marek Disease Vaccines)
SB  - IM
MH  - Aging
MH  - Animals
MH  - Chickens
MH  - DNA, Viral/genetics
MH  - Dose-Response Relationship, Immunologic
MH  - Genes, Viral
MH  - Injections, Subcutaneous
MH  - Lung/immunology/*virology
MH  - Mardivirus/genetics/*physiology
MH  - Marek Disease/immunology/prevention & control
MH  - Marek Disease Vaccines/administration & dosage/*immunology
MH  - Viral Load
MH  - *Virus Replication
EDAT- 2010/04/15 06:00
MHDA- 2010/07/07 06:00
CRDT- 2010/04/15 06:00
PHST- 2010/04/15 06:00 [entrez]
PHST- 2010/04/15 06:00 [pubmed]
PHST- 2010/07/07 06:00 [medline]
AID - 921016677 [pii]
AID - 10.1080/03079451003599250 [doi]
PST - ppublish
SO  - Avian Pathol. 2010 Apr;39(2):71-9. doi: 10.1080/03079451003599250.