PMID- 20390923
OWN - NLM
STAT- MEDLINE
DCOM- 20100701
LR  - 20160526
IS  - 1532-4117 (Electronic)
IS  - 1093-4529 (Linking)
VI  - 45
IP  - 6
DP  - 2010
TI  - Microbial community structure and population dynamics of granules developed in 
      expanded granular sludge bed (EGSB) reactors for the anaerobic treatment of 
      low-strength wastewater at low temperature.
PG  - 754-66
LID - 10.1080/10934521003651531 [doi]
AB  - The anaerobic biological treatment of sucrose-based, low-strength wastewater was 
      investigated in expanded granular sludge bed (EGSB) reactors at low temperatures 
      over a 300-day trial period. During the trial, the operating temperature was 
      lowered in a stepwise manner from 20 degrees C to 5 degrees C. As a result, the 
      reactors exhibited sufficient performances until 10 degrees C operation. The COD 
      removal rate was 3.1-3.8 kgCOD m(-3) day(-1) at 10 degrees C. In particular, the 
      COD removal rate increased gradually through the low-temperature operation; 
      indeed, the later stages of the 10 degrees C operation attained a rate similar to 
      those achieved at 20 degrees C and 15 degrees C. This finding is especially 
      practical for applications of psychrophilic methane fermentation. Additionally, 
      the structure of the microbial community in the granular sludge was analyzed by 
      clone analysis based on 16S rRNA genes and fluorescence in situ hybridization 
      (FISH). As a result, the percentage of the phylum Firmicutes, which were assumed 
      to be Anaerobivrio sp. and Lactococcus sp., greatly increased from 0.7% to 8.0% 
      of the total cells, especially in the surface layer of the granular sludge. These 
      bacteria would contribute to the degradation of the sucrose substrate 
      anaerobically at ambient temperatures. Moreover, the results suggest that a 
      Methanospirillum species, which is a H2-utilizing methanogen, increased from 0.5% 
      to 6.7% during the low-temperature incubation, with a significant increase of 
      methanogenic activity from H2/CO2 at 20 degrees C. Thus, the Methanospirillum 
      species detected in this study may have a key role as hydrogen scavenger during 
      hydrogen-metabolism in low-temperature conditions.
FAU - Tsushima, Ikuo
AU  - Tsushima I
AD  - Water and Soil Environment Division, National Institute for Environmental Studies 
      (NIES), Ibaraki, Japan.
FAU - Yoochatchaval, Wilasinee
AU  - Yoochatchaval W
FAU - Yoshida, Hiroki
AU  - Yoshida H
FAU - Araki, Nobuo
AU  - Araki N
FAU - Syutsubo, Kazuaki
AU  - Syutsubo K
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - England
TA  - J Environ Sci Health A Tox Hazard Subst Environ Eng
JT  - Journal of environmental science and health. Part A, Toxic/hazardous substances & 
      environmental engineering
JID - 9812551
RN  - 0 (DNA Probes)
RN  - 0 (Sewage)
SB  - IM
MH  - *Anaerobiosis
MH  - Base Sequence
MH  - *Bioreactors
MH  - *Cold Temperature
MH  - DNA Probes
MH  - In Situ Hybridization
MH  - Phylogeny
MH  - Polymerase Chain Reaction
MH  - Sewage/*microbiology
EDAT- 2010/04/15 06:00
MHDA- 2010/07/02 06:00
CRDT- 2010/04/15 06:00
PHST- 2010/04/15 06:00 [entrez]
PHST- 2010/04/15 06:00 [pubmed]
PHST- 2010/07/02 06:00 [medline]
AID - 921253308 [pii]
AID - 10.1080/10934521003651531 [doi]
PST - ppublish
SO  - J Environ Sci Health A Tox Hazard Subst Environ Eng. 2010;45(6):754-66. doi: 
      10.1080/10934521003651531.