PMID- 20393578
OWN - NLM
STAT- MEDLINE
DCOM- 20100713
LR  - 20211020
IS  - 1744-4292 (Electronic)
IS  - 1744-4292 (Linking)
VI  - 6
DP  - 2010 Apr 13
TI  - Transcript counting in single cells reveals dynamics of rDNA transcription.
PG  - 358
LID - 10.1038/msb.2010.14 [doi]
AB  - Most eukaryotes contain many tandem repeats of ribosomal RNA genes of which only 
      a subset is transcribed at any given time. Current biochemical methods allow for 
      the determination of the fraction of transcribing repeats (ON) versus 
      non-transcribing repeats (OFF) but do not provide any dynamical information and 
      obscure any transcription activity at the single-cell level. Here, we use a 
      fluorescence in situ hybridization (FISH) technique that allows the detection of 
      single-RNA molecules in individual yeast cells. We use this method complemented 
      with theoretical modeling to determine the rate of switching from OFF to ON 
      (activation rate) and the average number of RNA molecules produced during each 
      transcriptional burst (burst size). We explore how these two variables change in 
      mutants and different growth conditions, and show that this method resolves 
      changes in these two variables even when the average rDNA expression is 
      unaltered. These phenotypic changes could not have been detected by traditional 
      biochemical assays.
FAU - Tan, Rui Zhen
AU  - Tan RZ
AD  - Harvard University Graduate Biophysics Program, Harvard Medical School, Boston, 
      MA, USA.
FAU - van Oudenaarden, Alexander
AU  - van Oudenaarden A
LA  - eng
GR  - R01 GM068957/GM/NIGMS NIH HHS/United States
GR  - R01-GM068957/GM/NIGMS NIH HHS/United States
PT  - Journal Article
PT  - Research Support, N.I.H., Extramural
PT  - Research Support, Non-U.S. Gov't
PT  - Research Support, U.S. Gov't, Non-P.H.S.
PL  - England
TA  - Mol Syst Biol
JT  - Molecular systems biology
JID - 101235389
RN  - 0 (DNA, Ribosomal)
RN  - 0 (Fungal Proteins)
RN  - 0 (RNA, Fungal)
RN  - 0 (RNA, Messenger)
RN  - EC 3.5.1.98 (Histone Deacetylases)
SB  - IM
MH  - DNA, Ribosomal/*genetics/metabolism
MH  - Fungal Proteins/genetics/metabolism
MH  - Genes, Fungal
MH  - Histone Deacetylases/genetics/metabolism
MH  - In Situ Hybridization, Fluorescence
MH  - Microscopy
MH  - *Models, Genetic
MH  - RNA, Fungal/genetics/metabolism
MH  - RNA, Messenger/genetics/metabolism
MH  - *Transcription, Genetic
MH  - Yeasts/cytology/genetics/metabolism
PMC - PMC2872610
COIS- The authors declare that they have no conflict of interest.
EDAT- 2010/04/16 06:00
MHDA- 2010/07/14 06:00
PMCR- 2010/04/13
CRDT- 2010/04/16 06:00
PHST- 2009/08/14 00:00 [received]
PHST- 2010/02/09 00:00 [accepted]
PHST- 2010/04/16 06:00 [entrez]
PHST- 2010/04/16 06:00 [pubmed]
PHST- 2010/07/14 06:00 [medline]
PHST- 2010/04/13 00:00 [pmc-release]
AID - msb201014 [pii]
AID - 10.1038/msb.2010.14 [doi]
PST - ppublish
SO  - Mol Syst Biol. 2010 Apr 13;6:358. doi: 10.1038/msb.2010.14.