PMID- 20393974 OWN - NLM STAT- MEDLINE DCOM- 20100624 LR - 20221222 IS - 1934-3663 (Electronic) IS - 1934-3655 (Linking) VI - Chapter 25 DP - 2010 Apr TI - PRIDE: Data submission and analysis. PG - 25.4.1-25.4.11 LID - 10.1002/0471140864.ps2504s60 [doi] AB - The Proteomics Identifications database (PRIDE, http://www.ebi.ac.uk/pride) is one of the main repositories designed to store, disseminate, and analyze mass spectrometry-based proteomics datasets. In this unit, an overview of the PRIDE system is given, including its key satellite tools: the Ontology Lookup Service (OLS), the Protein Identifier Cross-Referencing Service (PICR), and Database on Demand (DoD). Also described in detail are procedures for submitting data to PRIDE, and accessing data stored in PRIDE using the BioMart interface. Finally, to demonstrate the potential of PRIDE as a source for data mining, an example protocol is provided to showcase the powerful cross-domain query capabilities available through a combination of BioMarts. FAU - Vizcaino, Juan Antonio AU - Vizcaino JA AD - European Molecular Biology Laboratory-European Bioinformatics Institute (EMBL-EBI), Hinxton, Cambridge, United Kingdom. FAU - Reisinger, Florian AU - Reisinger F AD - European Molecular Biology Laboratory-European Bioinformatics Institute (EMBL-EBI), Hinxton, Cambridge, United Kingdom. FAU - Cote, Richard AU - Cote R AD - European Molecular Biology Laboratory-European Bioinformatics Institute (EMBL-EBI), Hinxton, Cambridge, United Kingdom. FAU - Martens, Lennart AU - Martens L AD - European Molecular Biology Laboratory-European Bioinformatics Institute (EMBL-EBI), Hinxton, Cambridge, United Kingdom. LA - eng PT - Journal Article PL - United States TA - Curr Protoc Protein Sci JT - Current protocols in protein science JID - 101287868 SB - IM MH - Database Management Systems MH - *Databases, Protein MH - Internet MH - Mass Spectrometry MH - Proteomics/*methods MH - Software MH - *User-Computer Interface EDAT- 2010/04/16 06:00 MHDA- 2010/06/25 06:00 CRDT- 2010/04/16 06:00 PHST- 2010/04/16 06:00 [entrez] PHST- 2010/04/16 06:00 [pubmed] PHST- 2010/06/25 06:00 [medline] AID - 10.1002/0471140864.ps2504s60 [doi] PST - ppublish SO - Curr Protoc Protein Sci. 2010 Apr;Chapter 25:25.4.1-25.4.11. doi: 10.1002/0471140864.ps2504s60.