PMID- 20394576
OWN - NLM
STAT- MEDLINE
DCOM- 20101221
LR  - 20190911
IS  - 1875-5305 (Electronic)
IS  - 0929-8665 (Linking)
VI  - 17
IP  - 9
DP  - 2010 Sep
TI  - IgE binding epitopes of Bla g 6 from German cockroach.
PG  - 1170-6
AB  - Bla g 6, a German cockroach allergen, shows homology to muscle protein troponin 
      C. It contains four calcium-binding domains at amino acid (aa) residues 20-30, 
      56-67, 96-107, and 132-143, and its immunoglobulin E (IgE) reactivity is 
      dependent upon calcium ion level. However, the IgE binding epitopes of Bla g 6 
      have not been investigated. This study aimed to analyze the IgE binding epitopes 
      from the five peptide fragments of Bla g 6. The full-length of three Bla g 6 
      isoallergens (Bla g 6.0101, Bla g 6.0201, and Bla g 6.0301) and five peptide 
      fragments (P1: aa 1-111, P2: aa 1-95, P3: aa 33-111, P4: aa 80-151, and P5: aa 
      33-151) of Bla g 6.0101 were generated by polymerase chain reaction (PCR) and 
      expressed in Escherichia coli. Enzyme-linked immunosorbent assay (ELISA) was 
      performed on 24 patients' sera that adjusted the final concentration 10 mM of 
      CaCl(2) to determine the IgE activities of Bla g 6. Eight sera (33.3%), 9 sera 
      (37.5%), and 11 sera (45.8%) showed IgE reactivity to Bla g 6.0101, Bla g 6.0201, 
      and Bla g 6.0301, respectively. Among the sera from the positive IgE reactivity, 
      three patients' sera were selected and the IgE reactivity was measured by ELISA 
      with the five peptide fragments of Bla g 6. Based on IgE responses, one patient's 
      serum exhibited the strongest IgE reactivity. We assumed that the aa between 
      96-151 residues, including the calcium binding domains III and IV, would be 
      important for IgE binding. These results may provide information that will yield 
      safe diagnostic methods and immunotherapeutics.
FAU - Un, Sunjin
AU  - Un S
AD  - Department of Environmental Medical Biology and Institute of Tropical Medicine, 
      Korea National Arthropods of Medical Importance Resource Bank, Brain Korea 21 
      Project for Medical Science, Yonsei University College of Medicine, Seoul, Korea.
FAU - Jeong, Kyoung Yong
AU  - Jeong KY
FAU - Yi, Myung-hee
AU  - Yi MH
FAU - Kim, Chung-ryul
AU  - Kim CR
FAU - Yong, Tai-Soon
AU  - Yong TS
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - Netherlands
TA  - Protein Pept Lett
JT  - Protein and peptide letters
JID - 9441434
RN  - 0 (Allergens)
RN  - 0 (Calcium-Binding Proteins)
RN  - 0 (Epitopes)
RN  - 0 (Troponin C)
RN  - 37341-29-0 (Immunoglobulin E)
SB  - IM
MH  - Adolescent
MH  - Adult
MH  - Allergens/chemistry/immunology/metabolism
MH  - Animals
MH  - Calcium-Binding Proteins/metabolism
MH  - Child
MH  - Cockroaches/*immunology
MH  - Epitopes/chemistry/*immunology/*metabolism
MH  - Female
MH  - Humans
MH  - Immunoglobulin E/*immunology/*metabolism
MH  - Male
MH  - Middle Aged
MH  - Troponin C/metabolism
MH  - Young Adult
EDAT- 2010/04/17 06:00
MHDA- 2010/12/22 06:00
CRDT- 2010/04/17 06:00
PHST- 2009/11/17 00:00 [received]
PHST- 2010/03/18 00:00 [accepted]
PHST- 2010/04/17 06:00 [entrez]
PHST- 2010/04/17 06:00 [pubmed]
PHST- 2010/12/22 06:00 [medline]
AID - BSP/ PPL/ E pub/0141 [pii]
AID - 10.2174/092986610791760432 [doi]
PST - ppublish
SO  - Protein Pept Lett. 2010 Sep;17(9):1170-6. doi: 10.2174/092986610791760432.