PMID- 20394810
OWN - NLM
STAT- MEDLINE
DCOM- 20100527
LR  - 20181121
IS  - 1879-3169 (Electronic)
IS  - 0378-4274 (Linking)
VI  - 196
IP  - 2
DP  - 2010 Jul 1
TI  - Effects of acute in vitro exposure of murine precision-cut lung slices to gaseous 
      nitrogen dioxide and ozone in an air-liquid interface (ALI) culture.
PG  - 117-24
LID - 10.1016/j.toxlet.2010.04.004 [doi]
AB  - The aim of this study was to establish an air-liquid interface (ALI) culture of 
      precision-cut lung slices (PCLS) for direct exposure of lung cells to gaseous 
      contaminants. Nitrogen dioxide (NO(2)) and ozone (O(3)) were selected as model 
      gas compounds. Acute pro-inflammatory and toxic effects of NO(2) and O(3) on live 
      lung tissue were investigated. Murine PCLS were exposed to different flow rates 
      (3-30mL/min) of synthetic air, O(3) (3.5-8.5ppm), or NO(2) (1-80ppm). Tissue 
      survived ex vivo in ALI culture and resisted exposure to NO(2) (1-10ppm) and O(3) 
      (3.5-8.5ppm) for 1h. Longer exposure to NO(2) resulted in a clear loss of 
      viability, whereas exposure to O(3) was less effective. Exposure to NO(2) 
      dose-dependently induced release of the pro-inflammatory IL-1alpha (40%), whereas 
      RANTES, IL-12, and eotaxin remained unchanged. Early secretion of IL-1alpha 
      (80%), RANTES (>800%), MIP-1beta (44%), and MCP-1 (60%) was already detected 
      after 1h of exposure to O(3). The obtained data showed that direct exposure to 
      O(3) and NO(2) induced cytotoxicity and pro-inflammatory responses in PCLS with 
      ALI culture. This provides a model that more closely resembles in vivo exposure 
      of airborne contaminants, and thus should be appropriate for toxicity testing.
FAU - Switalla, S
AU  - Switalla S
AD  - Fraunhofer Institute for Toxicology and Experimental Medicine, Nikolai-Fuchs-Str. 
      1, 30625 Hannover, Germany.
FAU - Knebel, J
AU  - Knebel J
FAU - Ritter, D
AU  - Ritter D
FAU - Krug, N
AU  - Krug N
FAU - Braun, A
AU  - Braun A
FAU - Sewald, K
AU  - Sewald K
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20100413
PL  - Netherlands
TA  - Toxicol Lett
JT  - Toxicology letters
JID - 7709027
RN  - 0 (Ccl2 protein, mouse)
RN  - 0 (Ccl4 protein, mouse)
RN  - 0 (Ccl5 protein, mouse)
RN  - 0 (Chemokine CCL2)
RN  - 0 (Chemokine CCL4)
RN  - 0 (Chemokine CCL5)
RN  - 0 (Gases)
RN  - 0 (Inflammation Mediators)
RN  - 0 (Interleukin-1alpha)
RN  - 0 (Lipopolysaccharides)
RN  - 0 (lipopolysaccharide, Escherichia coli O111 B4)
RN  - 187348-17-0 (Interleukin-12)
RN  - 66H7ZZK23N (Ozone)
RN  - S7G510RUBH (Nitrogen Dioxide)
SB  - IM
MH  - Animals
MH  - Chemokine CCL2/metabolism
MH  - Chemokine CCL4/metabolism
MH  - Chemokine CCL5/metabolism
MH  - Dose-Response Relationship, Drug
MH  - Female
MH  - Gases
MH  - Inflammation Mediators/metabolism
MH  - Interleukin-12/metabolism
MH  - Interleukin-1alpha/metabolism
MH  - Lipopolysaccharides/pharmacology
MH  - Lung/*drug effects/immunology/pathology
MH  - Mice
MH  - Mice, Inbred BALB C
MH  - Nitrogen Dioxide/*toxicity
MH  - Ozone/*toxicity
MH  - Time Factors
MH  - Tissue Culture Techniques
MH  - Tissue Survival/drug effects
EDAT- 2010/04/17 06:00
MHDA- 2010/05/28 06:00
CRDT- 2010/04/17 06:00
PHST- 2010/01/01 00:00 [received]
PHST- 2010/03/12 00:00 [revised]
PHST- 2010/04/07 00:00 [accepted]
PHST- 2010/04/17 06:00 [entrez]
PHST- 2010/04/17 06:00 [pubmed]
PHST- 2010/05/28 06:00 [medline]
AID - S0378-4274(10)01309-3 [pii]
AID - 10.1016/j.toxlet.2010.04.004 [doi]
PST - ppublish
SO  - Toxicol Lett. 2010 Jul 1;196(2):117-24. doi: 10.1016/j.toxlet.2010.04.004. Epub 
      2010 Apr 13.