PMID- 20396870
OWN - NLM
STAT- MEDLINE
DCOM- 20110418
LR  - 20131121
IS  - 1432-0738 (Electronic)
IS  - 0340-5761 (Linking)
VI  - 85
IP  - 1
DP  - 2011 Jan
TI  - Perinatal cisplatin exposure induces cochlear apoptosis in newborn guinea pigs.
PG  - 19-25
LID - 10.1007/s00204-010-0543-7 [doi]
AB  - The objective of this study was to evaluate the role of apoptosis in the 
      development of the newborn cochlear structures and hearing loss caused by 
      prenatal cis-diaminedichloroplatinum (cisplatin) exposure. Pregnant albino guinea 
      pigs were intraperitoneally injected with 1.5 mg/kg body weight cisplatin once a 
      day for seven consecutive days at gestational day (GD) 51 to GD 57. At postnatal 
      day (PND) 14, pups were examined in the distortion product otoacoustic emission 
      (DPOAE) task. The temporal bones were then removed and immunohistochemically 
      stained for caspase 3, using the terminal deoxynucleotidyl transferase-mediated 
      dUTP-biotin nick end-labeling (TUNEL) method. Cisplatin used during pregnancy 
      could induce hearing loss in newborn and cochlear hair cell apoptosis. In 
      conclusion, apoptosis may play an important role in the development of hearing 
      impairment, caused by perinatal cisplatin exposure.
FAU - Hao, Shuai
AU  - Hao S
AD  - Department of Otolaryngology, First Affiliated Hospital of China Medical 
      University, Shenyang, Liaoning, People's Republic of China.
FAU - Jiang, Xuejun
AU  - Jiang X
FAU - Yan, Aihui
AU  - Yan A
FAU - Yang, Bo
AU  - Yang B
LA  - eng
PT  - Journal Article
DEP - 20100416
PL  - Germany
TA  - Arch Toxicol
JT  - Archives of toxicology
JID - 0417615
RN  - EC 3.4.22.- (Caspase 3)
RN  - Q20Q21Q62J (Cisplatin)
SB  - IM
MH  - Animals
MH  - Animals, Newborn
MH  - *Apoptosis
MH  - Caspase 3/metabolism
MH  - Cisplatin/administration & dosage/*toxicity
MH  - Cochlea/embryology/*pathology
MH  - Female
MH  - Guinea Pigs
MH  - Hearing Loss/*chemically induced
MH  - Immunohistochemistry
MH  - In Situ Nick-End Labeling
MH  - Injections, Intraperitoneal
MH  - Male
MH  - Otoacoustic Emissions, Spontaneous
MH  - Pregnancy
MH  - *Prenatal Exposure Delayed Effects
EDAT- 2010/04/17 06:00
MHDA- 2011/04/19 06:00
CRDT- 2010/04/17 06:00
PHST- 2009/11/08 00:00 [received]
PHST- 2010/04/01 00:00 [accepted]
PHST- 2010/04/17 06:00 [entrez]
PHST- 2010/04/17 06:00 [pubmed]
PHST- 2011/04/19 06:00 [medline]
AID - 10.1007/s00204-010-0543-7 [doi]
PST - ppublish
SO  - Arch Toxicol. 2011 Jan;85(1):19-25. doi: 10.1007/s00204-010-0543-7. Epub 2010 Apr 
      16.