PMID- 2039822
OWN - NLM
STAT- MEDLINE
DCOM- 19910710
LR  - 20210216
IS  - 0006-4971 (Print)
IS  - 0006-4971 (Linking)
VI  - 77
IP  - 11
DP  - 1991 Jun 1
TI  - Detection and characterization of human T-cell lymphotropic virus type I (HTLV-I) 
      associated T-cell neoplasms in an HTLV-I nonendemic region by polymerase chain 
      reaction.
PG  - 2419-30
AB  - Human T-cell lymphotropic virus type I (HTLV-I) associated adult T-cell 
      leukemia/lymphoma (ATLL) occurs endemically in southwestern Japan, the Caribbean, 
      and West Africa, but occurs sporadically in most of the rest of the world. 
      However, because ATLL and non-HTLV-I associated T-cell neoplasms share 
      overlapping clinicopathologic features, the prevalence of ATLL in nonendemic 
      regions is unknown. In this study, 75 T-cell neoplasms randomly procured from the 
      metropolitan New York City area were examined by polymerase chain reaction (PCR) 
      for the presence of integrated HTLV-I proviral sequences. HTLV-I genomic 
      sequences were detected by PCR in 6 of the 75 cases (8%); this result was 
      confirmed by Southern blot hybridization. The clinicopathologic features of the 
      HTLV-I positive and HTLV-I negative T-cell neoplasms were then compared. Although 
      the clinicopathologic features of patients from these two groups overlapped, some 
      findings were more commonly associated with HTLV-I positive neoplasms. Five of 
      the six patients with HTLV-I positive neoplasms were from HTLV-I endemic areas, 
      five were black, five were women, and five were less than 45 years of age, while 
      the majority of the patients with HTLV-I negative T-cell malignancies were 
      elderly white men. The incidence of hypercalcemia and lytic bone lesions was 
      significantly more common among patients with HTLV-I positive T-cell neoplasms (P 
      less than .001 and P = .004, respectively). The immunophenotypes of the HTLV-I 
      positive and negative tumors were similar; however, all HTLV-I positive neoplasms 
      were CD7 negative (P less than .001). In summary, our findings: (1) demonstrate 
      the special clinicopathologic and immunophenotypic features of HTLV-I positive 
      T-cell neoplasms, (2) suggest that most of the rare cases of HTLV-I-associated 
      T-cell neoplasms occurring in HTLV-I nonendemic areas are actually endemic cases; 
      and (3) that PCR is a sensitive, clinically useful technique for identifying 
      HTLV-I associated T-cell neoplasms.
FAU - Chadburn, A
AU  - Chadburn A
AD  - Department of Pathology, Columbia University College of Physicians and Surgeons, 
      New York, NY 10032.
FAU - Athan, E
AU  - Athan E
FAU - Wieczorek, R
AU  - Wieczorek R
FAU - Knowles, D M
AU  - Knowles DM
LA  - eng
GR  - CA48236/CA/NCI NIH HHS/United States
GR  - EY06337/EY/NEI NIH HHS/United States
PT  - Journal Article
PT  - Research Support, U.S. Gov't, P.H.S.
PL  - United States
TA  - Blood
JT  - Blood
JID - 7603509
RN  - 0 (Antigens, CD)
RN  - 0 (DNA, Viral)
RN  - 0 (Oligonucleotide Probes)
SB  - IM
MH  - Adult
MH  - Antigens, CD/analysis
MH  - Base Sequence
MH  - Blotting, Southern
MH  - DNA, Viral/genetics/isolation & purification
MH  - Female
MH  - Genes, Viral
MH  - Human T-lymphotropic virus 1/genetics/*isolation & purification
MH  - Humans
MH  - Leukemia, T-Cell/*microbiology/pathology
MH  - Leukemia-Lymphoma, Adult T-Cell/epidemiology/*microbiology/pathology
MH  - Lymphoma, T-Cell/*microbiology/pathology
MH  - Male
MH  - Molecular Sequence Data
MH  - New York City
MH  - Oligonucleotide Probes
MH  - Polymerase Chain Reaction/*methods
MH  - Prevalence
EDAT- 1991/06/01 00:00
MHDA- 1991/06/01 00:01
CRDT- 1991/06/01 00:00
PHST- 1991/06/01 00:00 [pubmed]
PHST- 1991/06/01 00:01 [medline]
PHST- 1991/06/01 00:00 [entrez]
AID - S0006-4971(20)77830-4 [pii]
PST - ppublish
SO  - Blood. 1991 Jun 1;77(11):2419-30.