PMID- 20398362
OWN - NLM
STAT- MEDLINE
DCOM- 20100705
LR  - 20211020
IS  - 1471-2121 (Electronic)
IS  - 1471-2121 (Linking)
VI  - 11
DP  - 2010 Apr 16
TI  - Functional neural differentiation of human adipose tissue-derived stem cells 
      using bFGF and forskolin.
PG  - 25
LID - 10.1186/1471-2121-11-25 [doi]
AB  - BACKGROUND: Adult mesenchymal stem cells (MSCs) derived from adipose tissue have 
      the capacity to differentiate into mesenchymal as well as endodermal and 
      ectodermal cell lineage in vitro. We characterized the multipotent ability of 
      human adipose tissue-derived stem cells (hADSCs) as MSCs and investigated the 
      neural differentiation potential of these cells. RESULTS: Human ADSCs from 
      earlobe fat maintained self-renewing capacity and differentiated into adipocytes, 
      osteoblasts, or chondrocytes under specific culture conditions. Following neural 
      induction with bFGF and forskolin, hADSCs were differentiated into various types 
      of neural cells including neurons and glia in vitro. In neural 
      differentiated-hADSCs (NI-hADSCs), the immunoreactivities for neural stem cell 
      marker (nestin), neuronal markers (Tuj1, MAP2, NFL, NFM, NFH, NSE, and NeuN), 
      astrocyte marker (GFAP), and oligodendrocyte marker (CNPase) were significantly 
      increased than in the primary hADSCs. RT-PCR analysis demonstrated that the mRNA 
      levels encoding for ABCG2, nestin, Tuj1, MAP2, NFL, NFM, NSE, GAP43, SNAP25, 
      GFAP, and CNPase were also highly increased in NI-hADSCs. Moreover, NI-hADSCs 
      acquired neuron-like functions characterized by the display of voltage-dependent 
      tetrodotoxin (TTX)-sensitive sodium currents, outward potassium currents, and 
      prominent negative resting membrane potentials under whole-cell patch clamp 
      recordings. Further examination by RT-PCR showed that NI-hADSCs expressed high 
      level of ionic channel genes for sodium (SCN5A), potassium (MaxiK, Kv4.2, and 
      EAG2), and calcium channels (CACNA1C and CACNA1G), which were expressed 
      constitutively in the primary hADSCs. In addition, we demonstrated that Kv4.3 and 
      Eag1, potassium channel genes, and NE-Na, a TTX-sensitive sodium channel gene, 
      were highly induced following neural differentiation. CONCLUSIONS: These combined 
      results indicate that hADSCs have the same self-renewing capacity and 
      multipotency as stem cells, and can be differentiated into functional neurons 
      using bFGF and forskolin.
FAU - Jang, Sujeong
AU  - Jang S
AD  - Department of Physiology, Chonnam National University Medical School, Gwangju 
      501190, Republic of Korea.
FAU - Cho, Hyong-Ho
AU  - Cho HH
FAU - Cho, Yong-Bum
AU  - Cho YB
FAU - Park, Jong-Seong
AU  - Park JS
FAU - Jeong, Han-Seong
AU  - Jeong HS
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20100416
PL  - England
TA  - BMC Cell Biol
JT  - BMC cell biology
JID - 100966972
RN  - 1F7A44V6OU (Colforsin)
RN  - 62031-54-3 (Fibroblast Growth Factors)
SB  - IM
MH  - Adipose Tissue/*cytology
MH  - Adolescent
MH  - Cell Differentiation/*drug effects
MH  - Cell Separation
MH  - Cells, Cultured
MH  - Child
MH  - Child, Preschool
MH  - Colforsin/*pharmacology
MH  - Ear, External/cytology
MH  - Fibroblast Growth Factors/*metabolism
MH  - Humans
MH  - Neurons/*cytology
MH  - Pluripotent Stem Cells/cytology
MH  - Stem Cells/*cytology
MH  - Young Adult
PMC - PMC2867791
EDAT- 2010/04/20 06:00
MHDA- 2010/07/06 06:00
PMCR- 2010/04/16
CRDT- 2010/04/20 06:00
PHST- 2009/10/29 00:00 [received]
PHST- 2010/04/16 00:00 [accepted]
PHST- 2010/04/20 06:00 [entrez]
PHST- 2010/04/20 06:00 [pubmed]
PHST- 2010/07/06 06:00 [medline]
PHST- 2010/04/16 00:00 [pmc-release]
AID - 1471-2121-11-25 [pii]
AID - 10.1186/1471-2121-11-25 [doi]
PST - epublish
SO  - BMC Cell Biol. 2010 Apr 16;11:25. doi: 10.1186/1471-2121-11-25.