PMID- 20398463
OWN - NLM
STAT- MEDLINE
DCOM- 20110708
LR  - 20100419
IS  - 1003-0603 (Print)
IS  - 1003-0603 (Linking)
VI  - 22
IP  - 4
DP  - 2010 Apr
TI  - [The role of mesenteric lymph in pathogenesis of systemic inflammatory response 
      syndrome and systemic complications following severe acute pancreatitis in rats].
PG  - 206-9
AB  - OBJECTIVE: To observe the effect of mesenteric lymph duct ligation (MLDL) on 
      systemic inflammatory response syndrome (SIRS) and systemic complications in 
      severe acute pancreatitis (SAP) in rats. METHODS: Twenty-four Sprague-Dawley (SD) 
      male rats were randomly divided into sham operation group, SAP group, and 
      SAP+MLDL group, with 8 rats in each group. Sham operation group underwent 
      laparotomy only. SAP complicated with multiple organ injury model was reproduced 
      by retrograde injection of sodium taurocholate into the pancreatic duct in SD 
      rats. In SAP+MLDL group MLDL was performed before reproduction of SAP. Rats were 
      sacrificed at 24 hours after operation. The terminal ileum were collected for 
      examination under light microscope. The samples of pancreas, lung and liver were 
      collected for measuring the level of myeloperoxidase (MPO). The mesenteric lymph 
      nodes were harvested for standard bacteriologic culture. The blood levels of 
      diamine oxidase (DAO), D-lactate, endotoxin, tumor necrosis factor-alpha 
      (TNF-alpha) and interleukin-6 (IL-6) were also measured. RESULTS: The blood 
      levels of DAO [(0.64 + or - 0.17) kU/L vs. (0.37 + or - 0.07) kU/L], D-lactate 
      [(8.16 + or - 1.79) ng/L vs. (3.24 + or - 1.00) ng/L], endotoxin [(0.068 + or - 
      0.019) kEU/L vs. (0.033 + or - 0.009) kEU/L], TNF-alpha [(65.21 + or - 13.38) 
      ng/L vs. (22.16 + or - 5.04) ng/L] and IL-6 [(7.95 + or - 1.83) ng/L vs. (4.26 + 
      or - 1.23) ng/L] in SAP group were increased significantly comparing with those 
      in sham operation group (all P<0.01). The contents of pulmonary tissue [(9.07 + 
      or - 2.52) U/g vs. (4.38 + or - 1.29) U/g], pancreatic [(5.14 + or - 1.24) U/g 
      vs. (2.88 + or - 0.75) U/g] and liver [(6.36 + or - 1.63) U/g vs. (3.19 + or - 
      0.96) U/g] MPO were increased significantly in SAP group comparing with those in 
      sham operation group (all P<0.01). The rate of bacterial translocation to 
      mesenteric lymph nodes in SAP group was significantly higher than that of the 
      sham operation group (75.0% vs. 0, P< 0.01). Compared to SAP group, the contents 
      of DAO [(0.50 + or - 0.13) kU/L], D-lactate [(6.23 + or - 1.25) ng/L] , endotoxin 
      [(0.048 + or - 0.014) kEU/L], TNF-alpha [(48.50 + or - 13.23) ng/L] and IL-6 
      [(6.06 + or - 1.64) ng/L] were significantly decreased in SAP+MLDL group (P<0.05 
      or P<0.01). The levels of pulmonary [(6.58 + or - 1.96) U/g], pancreatic [(4.01 + 
      or - 1.05) U/g] and liver [(4.64 + or - 1.34) U/g] MPO, as well as the rate of 
      bacterial translocation to mesenteric lymph nodes (12.5%) were significantly 
      lower in SAP+MLDL group than those in SAP group (all P<0.05). CONCLUSION: MLDL 
      decreases bacteria/ endotoxin translocation and reduces the systemic inflammatory 
      response by interrupting the lymph flow from the injured gut into the 
      bloodstream. This procedure may protect the small intestine mucosal barrier 
      function and other organs by reducing the neutrophil aggregation and the release 
      of cytokines.
FAU - Zou, Zhong-dong
AU  - Zou ZD
AD  - Research Institute of General Surgery, Fuzhou General Hospital of Nanjing 
      Military Command, Fuzhou 350025, Fujian, China.
FAU - Zhang, Zai-zhong
AU  - Zhang ZZ
FAU - Wang, Lie
AU  - Wang L
FAU - Wang, Yu
AU  - Wang Y
FAU - Zheng, Guo-hua
AU  - Zheng GH
LA  - chi
PT  - English Abstract
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - China
TA  - Zhongguo Wei Zhong Bing Ji Jiu Yi Xue
JT  - Zhongguo wei zhong bing ji jiu yi xue = Chinese critical care medicine = Zhongguo 
      weizhongbing jijiuyixue
JID - 9887521
RN  - 0 (Interleukin-6)
RN  - 0 (Tumor Necrosis Factor-alpha)
SB  - IM
MH  - Animals
MH  - Disease Models, Animal
MH  - Interleukin-6/blood
MH  - Intestine, Small/pathology
MH  - Ligation
MH  - Lymph Nodes/microbiology
MH  - Lymphatic Vessels/*surgery
MH  - Male
MH  - Mesentery/microbiology
MH  - Pancreatitis/*complications/pathology
MH  - Rats
MH  - Rats, Sprague-Dawley
MH  - Systemic Inflammatory Response Syndrome/etiology/*prevention & control
MH  - Tumor Necrosis Factor-alpha/blood
EDAT- 2010/04/20 06:00
MHDA- 2011/07/09 06:00
CRDT- 2010/04/20 06:00
PHST- 2010/04/20 06:00 [entrez]
PHST- 2010/04/20 06:00 [pubmed]
PHST- 2011/07/09 06:00 [medline]
PST - ppublish
SO  - Zhongguo Wei Zhong Bing Ji Jiu Yi Xue. 2010 Apr;22(4):206-9.