PMID- 20401444
OWN - NLM
STAT- MEDLINE
DCOM- 20130124
LR  - 20100419
IS  - 0371-0874 (Print)
IS  - 0371-0874 (Linking)
VI  - 62
IP  - 2
DP  - 2010 Apr 25
TI  - [Cerebral lymphatic blockage aggravates apoptosis of cortical neurons after 
      subarachnoid hemorrhage in rats in vivo].
PG  - 109-14
AB  - This present study was performed to investigate the influence of cerebral 
      lymphatic blockage (CLB) on apoptosis of cortical neurons after subarachnoid 
      hemorrhage (SAH) in rats in vivo. Healthy adult Wistar rats were randomly divided 
      into normal control group, SAH group and SAH+CLB group. SAH model was made by 
      double injection of autologous blood into the cisterna magna. On the third day 
      after the second cisternal injection, morphological changes of cortical cells 
      were observed by hematoxylin-eosin (HE) combined with propidium iodide (PI) 
      staining. Terminal deoxynucleotidyl transferase-mediated dUTP nick end labeling 
      (TUNEL) method was applied to determine in situ apoptosis in the cerebral cortex. 
      Immunohistochemistry was conducted to detect the expression of Caspase-3 and 
      Bcl-2 in cortical neurons. HE and PI staining showed that cortical neurons of SAH 
      rats were partly shrinkable; the nuclei showed wavy, folded or wrinkled 
      appearance, and some nuclei had the shape of crescent. The cortical neurons in 
      SAH+CLB group distributed sparsely and the nuclear fragmentation, apoptotic 
      bodies were found, surrounded by the formation of vacuoles. The numbers of 
      TUNEL-positive cells in SAH group and SAH+CLB group were higher than that in the 
      normal control group, while the number in SAH+CLB group was significantly higher 
      than that in the SAH group. Caspase-3 expressions in SAH group and SAH+CLB group 
      were higher than that in the normal control group, while the expression in 
      SAH+CLB group was significantly higher than that in the SAH group. Bcl-2 
      expressions in SAH group and SAH+CLB group were higher than that in the normal 
      control group, while the expression in the SAH+CLB group was significantly lower 
      than that in SAH group. The results obtained suggest that CLB exacerbates the 
      apoptosis of cortical neurons in rats after SAH by up-regulating Caspase-3 
      expression and down-regulating Bcl-2 expression.
FAU - Wang, Xuan
AU  - Wang X
AD  - Key Laboratory of Cerebral Microcirculation, University of Shandong, Taishan 
      Medical College, Department of Neurology, Affiliated Hospital of Taishan Medical 
      College, Taian 271000, China.
FAU - Gao, Xiang-Dong
AU  - Gao XD
FAU - Gao, Bing
AU  - Gao B
FAU - Jia, Li-Li
AU  - Jia LL
FAU - Yang, Ming-Feng
AU  - Yang MF
FAU - Zhang, Yan-Bo
AU  - Zhang YB
FAU - Sun, Bao-Liang
AU  - Sun BL
LA  - chi
PT  - English Abstract
PT  - Journal Article
PL  - China
TA  - Sheng Li Xue Bao
JT  - Sheng li xue bao : [Acta physiologica Sinica]
JID - 20730130R
RN  - 0 (Proto-Oncogene Proteins c-bcl-2)
RN  - EC 3.4.22.- (Casp3 protein, rat)
RN  - EC 3.4.22.- (Caspase 3)
SB  - IM
MH  - Animals
MH  - Apoptosis/*physiology
MH  - Caspase 3/metabolism
MH  - Cerebral Cortex/*pathology
MH  - Female
MH  - Lymphatic Vessels/*injuries/pathology
MH  - Male
MH  - Neurons/*pathology
MH  - Proto-Oncogene Proteins c-bcl-2/metabolism
MH  - Rats
MH  - Rats, Wistar
MH  - Subarachnoid Hemorrhage/pathology/*physiopathology
EDAT- 2010/04/20 06:00
MHDA- 2013/01/25 06:00
CRDT- 2010/04/20 06:00
PHST- 2010/04/20 06:00 [entrez]
PHST- 2010/04/20 06:00 [pubmed]
PHST- 2013/01/25 06:00 [medline]
PST - ppublish
SO  - Sheng Li Xue Bao. 2010 Apr 25;62(2):109-14.