PMID- 20402231
OWN - NLM
STAT- MEDLINE
DCOM- 20100511
LR  - 20100420
IS  - 0177-5103 (Print)
IS  - 0177-5103 (Linking)
VI  - 89
IP  - 2
DP  - 2010 Mar 9
TI  - Gill erosion and herpesvirus in Crassostrea gigas cultured in Baja California, 
      Mexico.
PG  - 137-44
LID - 10.3354/dao02189 [doi]
AB  - Recurrent episodes of mortality of Crassostrea gigas cultured in northwestern 
      Mexico have been occurring since 1997. Previous studies on bacteria, protozoans, 
      and metazoans as presumptive causal agents have been inconclusive. However, 
      erosions in the marginal indentation of gills have been frequently observed in 
      oysters from areas affected by mortality events, and in 2000 those lesions were 
      associated with the detection of a herpes-like virus. The present study aimed to 
      describe the histological alterations of eroded gills and to determine whether 
      ostreid herpesvirus 1 (OsHV-1) or a related virus was associated with them using 
      in situ hybridization (ISH). Histology showed that gill filaments were fused. In 
      severe cases, deformation of the interlamellar junctions, swelling, and the loss 
      of water channels was observed. ISH analysis revealed the presence of OsHV-1 DNA 
      or a related virus in cells of the gills. Some labeled cells were large with dark 
      granules inside their cytoplasm. These cells were surrounded by infiltrating 
      hemocytes. Some cells interpreted as hemocytes were labeled and observed in 
      eroded and non-eroded areas of the gill. Large cells detected by ISH were also 
      observed by conventional histology with hematoxylin-eosin staining. Whether the 
      virus produces the erosions in the gills, or the erosions in the gills are 
      produced by an unknown condition and favor the presence of the virus, remains 
      unresolved. It is also not clear whether the lesions contribute to mortality.
FAU - Vasquez-Yeomans, Rebeca
AU  - Vasquez-Yeomans R
AD  - Centro de Investigacion Cientifica y de Educacion Superior de Ensenada, CICESE, 
      Km 107 Carretera Ensenada-Tijuana 3918 Zona Playitas, CP 22860, Ensenada, Baja 
      California, Mexico.
FAU - Garcia-Ortega, Mauricio
AU  - Garcia-Ortega M
FAU - Caceres-Martinez, Jorge
AU  - Caceres-Martinez J
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - Germany
TA  - Dis Aquat Organ
JT  - Diseases of aquatic organisms
JID - 8807037
SB  - IM
MH  - Animals
MH  - Aquaculture
MH  - Crassostrea/*virology
MH  - Gills/*pathology/*virology
MH  - Herpesviridae/classification/*isolation & purification
MH  - Mexico
EDAT- 2010/04/21 06:00
MHDA- 2010/05/12 06:00
CRDT- 2010/04/21 06:00
PHST- 2010/04/21 06:00 [entrez]
PHST- 2010/04/21 06:00 [pubmed]
PHST- 2010/05/12 06:00 [medline]
AID - 10.3354/dao02189 [doi]
PST - ppublish
SO  - Dis Aquat Organ. 2010 Mar 9;89(2):137-44. doi: 10.3354/dao02189.