PMID- 20402673
OWN - NLM
STAT- MEDLINE
DCOM- 20100512
LR  - 20100420
IS  - 1600-0463 (Electronic)
IS  - 0903-4641 (Linking)
VI  - 118
IP  - 4
DP  - 2010 Apr
TI  - Homing of transplanted bone marrow cells in livers of Schistosoma 
      mansoni-infected mice.
PG  - 277-87
LID - 10.1111/j.1600-0463.2010.02585.x [doi]
AB  - The efficiency of differentiation of bone marrow cells (BMCs) into hepatocytes in 
      vivo and its importance in physiopathological processes is still debated. Murine 
      schistosomiasis was used as a liver injury model and unfractionated male mice 
      BMCs were transplanted through intrahepatic injection into non-irradiated 
      Schistosoma mansoni-infected female mice on their 16th week post-infection. Two 
      weeks after bone marrow transplantation, mice were sacrificed on a weekly basis 
      until 10 weeks. Tracing of male donor-derived cells in female recipient mice 
      livers was carried out by the detection of Y chromosome expression by fluorescent 
      in situ hybridization (FISH) and also of chromodomain Y-linked (CDYL) protein by 
      indirect immunofluorescence (IF). Their transformation into hepatocytes was 
      studied by double labelling indirect IF using antibodies directed against CDYL 
      and mouse albumin. Histopathological and electron microscopic examinations 
      revealed the presence of small hepatocyte-like cells in the periportal tracts and 
      in between the hepatocytes facing the sinusoids. Donor-derived cells showing Y 
      chromosome by FISH and expressing CDYL protein by IF were recovered in the 
      infected transplanted livers. The initial number of these cells increased with 
      increased post-transplantation time. Cells were mainly localized in the periphery 
      of schistosoma granuloma. Few donor-derived cells appeared within the hepatic 
      parenchymal tissue and showed positivity for albumin secretion by double 
      labelling with IF. We suggest that transplanted bone marrow stem cells can 
      repopulate the Schistosoma-infected liver of immunocompetent mice. Their 
      differentiation is a complex event controlled by many factors and needs to be 
      further characterized extensively. The extent and type of liver injury and the 
      number of transplanted cells are important variables in the process of stem cell 
      engraftment and differentiation into functioning hepatic cells that still need to 
      be defined.
FAU - Elkhafif, Nagwa
AU  - Elkhafif N
AD  - Department of Electron Microscopy, Theodor Bilharz Research Institute, Giza, 
      Egypt. nagwa_elkhafif@hotmail.com
FAU - Voss, Bruno
AU  - Voss B
FAU - Hammam, Olfat
AU  - Hammam O
FAU - Yehia, Hoda
AU  - Yehia H
FAU - Mansy, Soheir
AU  - Mansy S
FAU - Akl, Maha
AU  - Akl M
FAU - Boehm, Sabine
AU  - Boehm S
FAU - Mahmoud, Soheir
AU  - Mahmoud S
FAU - El Bendary, Omnia
AU  - El Bendary O
FAU - El Fandy, Gihan
AU  - El Fandy G
LA  - eng
PT  - Journal Article
PL  - Denmark
TA  - APMIS
JT  - APMIS : acta pathologica, microbiologica, et immunologica Scandinavica
JID - 8803400
SB  - IM
MH  - Animals
MH  - *Bone Marrow Transplantation
MH  - Cell Movement/*physiology
MH  - Female
MH  - Fluorescent Antibody Technique
MH  - Hematopoietic Stem Cells/*cytology
MH  - In Situ Hybridization, Fluorescence
MH  - Liver/*cytology
MH  - Male
MH  - Mice
MH  - Schistosomiasis mansoni/*surgery
EDAT- 2010/04/21 06:00
MHDA- 2010/05/13 06:00
CRDT- 2010/04/21 06:00
PHST- 2010/04/21 06:00 [entrez]
PHST- 2010/04/21 06:00 [pubmed]
PHST- 2010/05/13 06:00 [medline]
AID - APM2585 [pii]
AID - 10.1111/j.1600-0463.2010.02585.x [doi]
PST - ppublish
SO  - APMIS. 2010 Apr;118(4):277-87. doi: 10.1111/j.1600-0463.2010.02585.x.