PMID- 20403141
OWN - NLM
STAT- MEDLINE
DCOM- 20101206
LR  - 20100728
IS  - 1399-0039 (Electronic)
IS  - 0001-2815 (Linking)
VI  - 76
IP  - 2
DP  - 2010 Aug
TI  - Solid phase HLA antibody detection technology--challenges in interpretation.
PG  - 87-95
LID - 10.1111/j.1399-0039.2010.01486.x [doi]
AB  - The introduction into routine diagnostic laboratories of solid phase assays for 
      human leukocyte antigen (HLA) antibody detection has resulted in the application 
      of new laboratory matching algorithms in clinical organ transplantation which 
      have improved pre-transplant detection of immunization, in turn resulting in 
      avoidance of rejection in many cases which until their introduction would not 
      have been possible using the historical complement dependent serological 
      techniques. There have been two generations of solid phase assays introduced into 
      routine practice, namely, the enzyme-linked immunosorbent assay (ELISA) technique 
      and the use of fluorescent beads with HLA molecules bound to their surface which 
      can either be used in conventional flow cytometry or in conjunction with Luminex 
      instrumentation, the latter having become the most popular approach. The use of 
      the fluorescent bead techniques has raised interesting questions both with 
      respect to technical performance and the interpretation of the results obtained. 
      The advantages of bead technology for HLA antibody determination and the 
      technical issues requiring resolution are the subject of this review.
FAU - Tait, B D
AU  - Tait BD
AD  - National Transplant Services, Australian Red Cross Blood Service, Melbourne, 
      Australia. bdtait@arcbs.redcross.org.au
FAU - Hudson, F
AU  - Hudson F
FAU - Brewin, G
AU  - Brewin G
FAU - Cantwell, L
AU  - Cantwell L
FAU - Holdsworth, R
AU  - Holdsworth R
LA  - eng
PT  - Journal Article
PT  - Review
DEP - 20100408
PL  - England
TA  - Tissue Antigens
JT  - Tissue antigens
JID - 0331072
RN  - 0 (HLA Antigens)
RN  - 0 (Histocompatibility Antigens Class I)
RN  - 0 (Immunoglobulin M)
RN  - 0 (Isoantibodies)
RN  - 0 (MHC class I-related chain A)
RN  - 0 (beta 2-Microglobulin)
RN  - 9007-36-7 (Complement System Proteins)
SB  - IM
MH  - Complement System Proteins/metabolism
MH  - Enzyme-Linked Immunosorbent Assay/methods
MH  - Fluorescent Antibody Technique/methods
MH  - HLA Antigens/*immunology
MH  - Histocompatibility Antigens Class I/immunology
MH  - Histocompatibility Testing
MH  - Humans
MH  - Immunity, Innate
MH  - Immunoassay/*methods
MH  - Immunoglobulin M/analysis/blood
MH  - Isoantibodies/*analysis/blood
MH  - Transplantation Immunology
MH  - beta 2-Microglobulin/blood/immunology
EDAT- 2010/04/21 06:00
MHDA- 2010/12/14 06:00
CRDT- 2010/04/21 06:00
PHST- 2010/04/21 06:00 [entrez]
PHST- 2010/04/21 06:00 [pubmed]
PHST- 2010/12/14 06:00 [medline]
AID - TAN1486 [pii]
AID - 10.1111/j.1399-0039.2010.01486.x [doi]
PST - ppublish
SO  - Tissue Antigens. 2010 Aug;76(2):87-95. doi: 10.1111/j.1399-0039.2010.01486.x. 
      Epub 2010 Apr 8.