PMID- 20404050
OWN - NLM
STAT- MEDLINE
DCOM- 20100520
LR  - 20131121
IS  - 1535-3699 (Electronic)
IS  - 1535-3699 (Linking)
VI  - 235
IP  - 3
DP  - 2010 Mar
TI  - Acute circuit-resistance exercise increases expression of lymphocyte 
      agouti-related protein in young women.
PG  - 326-34
LID - 10.1258/ebm.2009.009209 [doi]
AB  - Exercise-induced leukocytosis and lymphocytosis is accompanied by up-regulation 
      and down-regulation of hundreds of genes in white blood cells (WBCs). 
      Agouti-related protein (AgRP) is an orexigenic peptide secreted predominantly 
      from the arcuate nucleus in the hypothalamus. AgRP affects feeding behavior and 
      plays a role in energy and glucose homeostasis and adiposity. The purpose of the 
      study was to determine effects of circuit resistance exercise (CRE) (9 exercises, 
      25 s per exercise) at different intensities on peripheral blood lymphocyte (PBL) 
      AgRP mRNA expression and its concentrations in lymphocytes and plasma. 
      Twenty-five young female college students were randomly divided into five groups: 
      control, 40% 1-repetition maximum (1-RM), 60% 1-RM, 80% 1-RM and combined (40 + 
      60 + 80% 1-RM) loads. Peripheral blood mononuclear cells were isolated by a 
      lymphocyte density gradient centrifugation method for AgRP mRNA expression. 
      Lymphocyte ATP, glycogen, AgRP, growth hormone (GH), and plasma AgRP, GH and 
      glucose concentrations were measured. CRE increased AgRP mRNA lymphocyte 
      expression significantly (P < 0.0001) at all intensities. A higher and 
      significant (P < 0.01) increase was found in the 60% 1-RM group when compared 
      with the other groups. The CRE-induced lymphocyte AgRP expression was accompanied 
      by elevations in plasma AgRP, glucose and GH levels as well as higher WBCs, 
      lymphocytes and neutrophil counts. Lymphocyte AgRP and GH concentrations were 
      significantly reduced (P < 0.05). Lymphocyte ATP content was unchanged and 
      glycogen was reduced in the combined group but not in the other groups. Data 
      indicate that AgRP mRNA is expressed in PBLs and that CRE increases its 
      expression. Data also reveal that the expression of AgRP was accompanied with 
      higher plasma AgRP and GH concentrations. Findings suggest that AgRP may provide 
      an important signal in the immune environment and that the lymphocyte may be 
      considered as an extra-hypothalamic source of plasma AgRP following exercise 
      stress.
FAU - Ghanbari-Niaki, Abbass
AU  - Ghanbari-Niaki A
AD  - University of Mazandaran, Babolsar, Mazandaran. ghanbara@yahoo.ca
FAU - Saghebjoo, Marziyeh
AU  - Saghebjoo M
FAU - Rashid-Lamir, Amir
AU  - Rashid-Lamir A
FAU - Fathi, Rozita
AU  - Fathi R
FAU - Kraemer, Robert R
AU  - Kraemer RR
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - Switzerland
TA  - Exp Biol Med (Maywood)
JT  - Experimental biology and medicine (Maywood, N.J.)
JID - 100973463
RN  - 0 (Agouti-Related Protein)
RN  - 9002-72-6 (Growth Hormone)
RN  - 9005-79-2 (Glycogen)
RN  - IY9XDZ35W2 (Glucose)
SB  - IM
MH  - Adult
MH  - Agouti-Related Protein/*biosynthesis
MH  - Body Mass Index
MH  - *Exercise
MH  - Female
MH  - *Gene Expression Regulation
MH  - Glucose/metabolism
MH  - Glycogen/metabolism
MH  - Growth Hormone/metabolism
MH  - Humans
MH  - Leukocytes/metabolism
MH  - Leukocytes, Mononuclear/cytology
MH  - Lymphocytes/*metabolism
MH  - Neutrophils/metabolism
MH  - Random Allocation
EDAT- 2010/04/21 06:00
MHDA- 2010/05/21 06:00
CRDT- 2010/04/21 06:00
PHST- 2010/04/21 06:00 [entrez]
PHST- 2010/04/21 06:00 [pubmed]
PHST- 2010/05/21 06:00 [medline]
AID - 235/3/326 [pii]
AID - 10.1258/ebm.2009.009209 [doi]
PST - ppublish
SO  - Exp Biol Med (Maywood). 2010 Mar;235(3):326-34. doi: 10.1258/ebm.2009.009209.