PMID- 20404167
OWN - NLM
STAT- MEDLINE
DCOM- 20100608
LR  - 20211020
IS  - 1091-6490 (Electronic)
IS  - 0027-8424 (Print)
IS  - 0027-8424 (Linking)
VI  - 107
IP  - 18
DP  - 2010 May 4
TI  - Selective and site-specific mobilization of dermal dendritic cells and Langerhans 
      cells by Th1- and Th2-polarizing adjuvants.
PG  - 8334-9
LID - 10.1073/pnas.0912817107 [doi]
AB  - Dendritic cells (DCs) initiate and polarize adaptive immune responses toward 
      varying functional outcomes. By means of intravital two-photon microscopy, we 
      report that dermal dendritic cells (DDCs) and Langerhans cells (LCs) are 
      differentially mobilized during contact sensitization and by adjuvants such as 
      unmethylated CpG oligonucleotide (CpG) and LPS that induce T helper type 1 (Th1) 
      responses, or papain that induces T helper type 2 (Th2) responses. In ear pinna, 
      contact sensitization, CpG, LPS, and papain all mobilized DDCs in three distinct 
      phases: increased motility and dendritic probing, directed migration, and entry 
      into lymphatic vessels. During the same treatments, the adjacent LCs in ear pinna 
      remained immotile over a 48-hr period of observation. In contrast, footpads 
      lacked DDCs and Th1-polarizing adjuvants selectively induced a delayed 
      mobilization of LCs after 48 hr. Th1 polarization of CD4(+) T cells was 
      independent of the immunization site, whereas ear immunization favored Th2 
      polarization, correlating with site-specific DC distribution and dynamics. Our 
      results provide an initial description of peripheral DC dynamics in response to 
      adjuvants and imply that LC mobilization enhances a Th1 response and is not 
      sufficient to trigger a Th2 response, whereas mobilization of DDCs alone is 
      sufficient to trigger T-cell proliferation and to polarize initial T-cell 
      activation toward a Th2 response.
FAU - Sen, Debasish
AU  - Sen D
AD  - Department of Physiology and Biophysics and Institute for Immunology, University 
      of California, Irvine, CA 92697-4561, USA.
FAU - Forrest, Luette
AU  - Forrest L
FAU - Kepler, Thomas B
AU  - Kepler TB
FAU - Parker, Ian
AU  - Parker I
FAU - Cahalan, Michael D
AU  - Cahalan MD
LA  - eng
GR  - GM-41514/GM/NIGMS NIH HHS/United States
GR  - R01 GM048071/GM/NIGMS NIH HHS/United States
GR  - R37 GM048071/GM/NIGMS NIH HHS/United States
GR  - R01 GM041514/GM/NIGMS NIH HHS/United States
GR  - N01AI50019/AI/NIAID NIH HHS/United States
GR  - GM-48071/GM/NIGMS NIH HHS/United States
GR  - P30 CA062203/CA/NCI NIH HHS/United States
GR  - N01-AI-50019/AI/NIAID NIH HHS/United States
PT  - Journal Article
PT  - Research Support, N.I.H., Extramural
DEP - 20100419
PL  - United States
TA  - Proc Natl Acad Sci U S A
JT  - Proceedings of the National Academy of Sciences of the United States of America
JID - 7505876
RN  - 0 (CPG-oligonucleotide)
RN  - 0 (Lipopolysaccharides)
RN  - 0 (Oligodeoxyribonucleotides)
SB  - IM
MH  - Animals
MH  - Cell Movement
MH  - *Cell Polarity
MH  - Cell Proliferation
MH  - Coculture Techniques
MH  - Langerhans Cells/cytology/*immunology
MH  - Lipopolysaccharides/immunology
MH  - Lymphocyte Activation
MH  - Mice
MH  - Mice, Inbred C57BL
MH  - Oligodeoxyribonucleotides/immunology
MH  - Th1 Cells/*cytology/*immunology
MH  - Th2 Cells/*cytology/*immunology
PMC - PMC2889549
COIS- The authors declare no conflict of interest.
EDAT- 2010/04/21 06:00
MHDA- 2010/06/09 06:00
PMCR- 2010/11/04
CRDT- 2010/04/21 06:00
PHST- 2010/04/21 06:00 [entrez]
PHST- 2010/04/21 06:00 [pubmed]
PHST- 2010/06/09 06:00 [medline]
PHST- 2010/11/04 00:00 [pmc-release]
AID - 0912817107 [pii]
AID - 200912817 [pii]
AID - 10.1073/pnas.0912817107 [doi]
PST - ppublish
SO  - Proc Natl Acad Sci U S A. 2010 May 4;107(18):8334-9. doi: 
      10.1073/pnas.0912817107. Epub 2010 Apr 19.