PMID- 20406296
OWN - NLM
STAT- MEDLINE
DCOM- 20110315
LR  - 20181201
IS  - 1462-2920 (Electronic)
IS  - 1462-2912 (Linking)
VI  - 12
IP  - 9
DP  - 2010 Sep
TI  - High diversity of ammonia-oxidizing archaea in permanent and seasonal 
      oxygen-deficient waters of the eastern South Pacific.
PG  - 2450-65
LID - 10.1111/j.1462-2920.2010.02218.x [doi]
AB  - The community structure of putative aerobic ammonia-oxidizing archaea (AOA) was 
      explored in two oxygen-deficient ecosystems of the eastern South Pacific: the 
      oxygen minimum zone off Peru and northern Chile (11 degrees S-20 degrees S), where permanent 
      suboxic and low-ammonium conditions are found at intermediate depths, and the 
      continental shelf off central Chile (36 degrees S), where seasonal oxygen-deficient and 
      relatively high-ammonium conditions develop in the water column, particularly 
      during the upwelling season. The AOA community composition based on the ammonia 
      monooxygenase subunit A (amoA) genes changed according to the oxygen 
      concentration in the water column and the ecosystem studied, showing a higher 
      diversity in the seasonal low-oxygen waters. The majority of the archaeal amoA 
      genotypes was affiliated to the uncultured clusters A (64%) and B (35%), with 
      Cluster A AOA being mainly associated with higher oxygen and ammonium 
      concentrations and Cluster B AOA with permanent oxygen- and ammonium-poor waters. 
      Q-PCR assays revealed that AOA are an abundant community (up to 10(5) amoA copies 
      ml(-1) ), while bacterial amoA genes from beta proteobacteria were undetected. Our 
      results thus suggest that a diverse uncultured AOA community, for which, 
      therefore, we do not have any physiological information, to date, is an important 
      component of the nitrifying community in oxygen-deficient marine ecosystems, and 
      particularly in rich coastal upwelling ones.
CI  - (c) 2010 Society for Applied Microbiology and Blackwell Publishing Ltd.
FAU - Molina, Veronica
AU  - Molina V
AD  - Departamento de Oceanografia and Centro de Investigacion Oceanografica en el 
      Pacifico Sudoriental, Universidad de Concepcion, Casilla 160-C, Concepcion, 
      Chile. vemolina@udec.cl
FAU - Belmar, Lucy
AU  - Belmar L
FAU - Ulloa, Osvaldo
AU  - Ulloa O
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20100419
PL  - England
TA  - Environ Microbiol
JT  - Environmental microbiology
JID - 100883692
RN  - 0 (DNA, Archaeal)
RN  - 7664-41-7 (Ammonia)
RN  - EC 1.- (Oxidoreductases)
RN  - EC 1.7.3.- (ammonia monooxygenase)
RN  - N762921K75 (Nitrogen)
RN  - S88TT14065 (Oxygen)
SB  - IM
MH  - Ammonia/*metabolism
MH  - Archaea/classification/*genetics/metabolism
MH  - Betaproteobacteria/genetics
MH  - Chile
MH  - DNA, Archaeal/genetics
MH  - Genes, Archaeal
MH  - Genotype
MH  - Nitrogen/analysis
MH  - Oxidoreductases/genetics
MH  - Oxygen/analysis
MH  - Pacific Ocean
MH  - Peru
MH  - Phylogeny
MH  - Seawater/analysis/*microbiology
MH  - *Water Microbiology
EDAT- 2010/04/22 06:00
MHDA- 2011/03/16 06:00
CRDT- 2010/04/22 06:00
PHST- 2010/04/22 06:00 [entrez]
PHST- 2010/04/22 06:00 [pubmed]
PHST- 2011/03/16 06:00 [medline]
AID - EMI2218 [pii]
AID - 10.1111/j.1462-2920.2010.02218.x [doi]
PST - ppublish
SO  - Environ Microbiol. 2010 Sep;12(9):2450-65. doi: 10.1111/j.1462-2920.2010.02218.x. 
      Epub 2010 Apr 19.