PMID- 20406849
OWN - NLM
STAT- MEDLINE
DCOM- 20101028
LR  - 20100708
IS  - 1531-2267 (Electronic)
IS  - 1094-8341 (Linking)
VI  - 42
IP  - 2
DP  - 2010 Jul 7
TI  - Differential expression and activity of the porcine type I interferon family.
PG  - 248-58
LID - 10.1152/physiolgenomics.00198.2009 [doi]
AB  - Type I interferons (IFNs) are central to innate and adaptive immunity, and many 
      have unique developmental and physiological functions. However, in most species, 
      only two subtypes, IFN-alpha and IFN-beta, have been well studied. Because of the 
      increasing importance of zoonotic viral diseases and the use of pigs to address 
      human research questions, it is important to know the complete repertoire and 
      activity of porcine type I IFNs. Here we show that porcine type I IFNs comprise 
      at least 39 functional genes distributed along draft genomic sequences of 
      chromosomes 1 and 10. These functional IFN genes are classified into 17 IFN-alpha 
      subtypes, 11 IFN-delta subtypes, 7 IFN-omega subtypes, and single-subtype 
      subclasses of IFN-alphaomega, IFN-beta, IFN-epsilon, and IFN-kappa. We found that 
      porcine type I IFNs have diverse expression profiles and antiviral activities 
      against porcine reproductive and respiratory syndrome virus (PRRSV) and vesicular 
      stomatitis virus (VSV), with activity ranging from 0 to >10(5) U.ng(-1).ml(-1). 
      Whereas most IFN-alpha subtypes retained the greatest antiviral activity against 
      both PRRSV and VSV in porcine and MARC-145 cells, some IFN-delta and IFN-omega 
      subtypes, IFN-beta, and IFN-alphaomega differed in their antiviral activity based 
      on target cells and viruses. Several IFNs, including IFN-alpha7/11, IFN-delta2/7, 
      and IFN-omega4, exhibited minimal or no antiviral activity in the tested target 
      cell-virus systems. Thus comparative studies showed that antiviral activity of 
      porcine type I IFNs is virus- and cell-dependent, and IFN-alphas are positively 
      correlated with induction of MxA, an IFN-stimulated gene. Collectively, these 
      data provide fundamental genomic information for porcine type I IFNs, information 
      that is necessary for understanding porcine physiological and antiviral 
      responses.
FAU - Sang, Yongming
AU  - Sang Y
AD  - Departments of Anatomy and Physiology, College of Veterinary Medicine, Kansas 
      State University, Manhattan, KS 66506, USA.
FAU - Rowland, Raymond R R
AU  - Rowland RR
FAU - Hesse, Richard A
AU  - Hesse RA
FAU - Blecha, Frank
AU  - Blecha F
LA  - eng
PT  - Journal Article
PT  - Research Support, U.S. Gov't, Non-P.H.S.
DEP - 20100420
PL  - United States
TA  - Physiol Genomics
JT  - Physiological genomics
JID - 9815683
RN  - 0 (Interferon Type I)
SB  - IM
MH  - Amino Acid Sequence
MH  - Animals
MH  - Immunity, Innate/genetics
MH  - Interferon Type I/*genetics/immunology/metabolism
MH  - Molecular Sequence Data
MH  - Phylogeny
MH  - Sequence Alignment
MH  - Swine
MH  - Virus Diseases/genetics/immunology
EDAT- 2010/04/22 06:00
MHDA- 2010/10/29 06:00
CRDT- 2010/04/22 06:00
PHST- 2010/04/22 06:00 [entrez]
PHST- 2010/04/22 06:00 [pubmed]
PHST- 2010/10/29 06:00 [medline]
AID - physiolgenomics.00198.2009 [pii]
AID - 10.1152/physiolgenomics.00198.2009 [doi]
PST - ppublish
SO  - Physiol Genomics. 2010 Jul 7;42(2):248-58. doi: 
      10.1152/physiolgenomics.00198.2009. Epub 2010 Apr 20.