PMID- 20408960
OWN - NLM
STAT- MEDLINE
DCOM- 20110318
LR  - 20171116
IS  - 1440-1789 (Electronic)
IS  - 0919-6544 (Linking)
VI  - 30
IP  - 6
DP  - 2010 Dec
TI  - Utility of in situ demonstration of 1p loss and p53 overexpression in pathologic 
      diagnosis of oligodendroglial tumors.
PG  - 586-96
LID - 10.1111/j.1440-1789.2010.01116.x [doi]
AB  - To improve the diagnostic accuracy of oligodendroglial tumors and to find more 
      convenient parameters that could predict the cytogenetic status, oligodendroglial 
      and astrocytic tumors were cytogenetically and immunohistochemically 
      investigated. Materials included 22 oligodendroglial tumors (15 
      oligodendrogliomas and 7 oligoastrocytomas) and 20 astrocytic tumors. 1p loss was 
      examined with the fluorescence in situ hybridization (FISH) method. Expression of 
      GFAP, Olig2 and p53 was immunohistochemically investigated and co-localization of 
      GFAP and Olig2 was evaluated on double-immunostained sections. Furthermore, TP53 
      mutation analyses were carried out on three oligodendroglial tumors showing p53 
      protein overexpression with a direct sequence analysis. Our FISH studies 
      demonstrated 1p loss in 73% of oligodendroglial tumors (80% oligodendrogliomas 
      and 57% oligoastrocytomas) and in only 10% of astrocytic tumors. There were no 
      clear-cut morphologic differences between 1p-deleted and 1p-intact 
      oligodendroglial tumors. GFAP and Olig2 were expressed in most oligodendroglial 
      and astrocytic tumors, and their cellular localization was almost independent of 
      each other. Overexpression of p53 was observed in five oligodendroglial tumors, 
      all of which were 1p-intact. In comparison, 16 oligodendroglial tumors with 1p 
      deletion showed no overexpression of p53. TP53 missense mutations were detected 
      in three of the p53 overexpressed oligodendroglial tumors studied. Our results 
      suggest that 1p loss is almost specific to oligodendroglial tumors. Although the 
      prediction of 1p status based solely on the morphologic features seems to be 
      difficult, the immunohistochemistry for p53 is a useful tool in that p53 
      overexpression is closely related to the 1p-intact status in oligodendroglial 
      tumors.
CI  - (c) 2010 Japanese Society of Neuropathology.
FAU - Hirose, Takanori
AU  - Hirose T
AD  - Department of Pathology, Saitama Medical School, Moroyama, Iruma-gun, Saitama, 
      Japan. thirose@tph.gr.jp
FAU - Ishizawa, Keisuke
AU  - Ishizawa K
FAU - Shimada, Shio
AU  - Shimada S
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - Australia
TA  - Neuropathology
JT  - Neuropathology : official journal of the Japanese Society of Neuropathology
JID - 9606526
RN  - 0 (Basic Helix-Loop-Helix Transcription Factors)
RN  - 0 (Glial Fibrillary Acidic Protein)
RN  - 0 (Nerve Tissue Proteins)
RN  - 0 (OLIG2 protein, human)
RN  - 0 (Oligodendrocyte Transcription Factor 2)
RN  - 0 (Tumor Suppressor Protein p53)
SB  - IM
MH  - Adult
MH  - Aged
MH  - Astrocytoma/*diagnosis/genetics/metabolism
MH  - Basic Helix-Loop-Helix Transcription Factors/biosynthesis
MH  - Brain Neoplasms/*diagnosis/genetics/metabolism
MH  - Chromosomes, Human, Pair 1/*genetics
MH  - Female
MH  - Gene Deletion
MH  - Glial Fibrillary Acidic Protein/biosynthesis
MH  - Humans
MH  - Immunohistochemistry
MH  - In Situ Hybridization, Fluorescence
MH  - Male
MH  - Middle Aged
MH  - Mutation
MH  - Nerve Tissue Proteins/biosynthesis
MH  - Oligodendrocyte Transcription Factor 2
MH  - Oligodendroglioma/*diagnosis/genetics/metabolism
MH  - Tumor Suppressor Protein p53/*biosynthesis/genetics
EDAT- 2010/04/23 06:00
MHDA- 2011/03/19 06:00
CRDT- 2010/04/23 06:00
PHST- 2010/04/23 06:00 [entrez]
PHST- 2010/04/23 06:00 [pubmed]
PHST- 2011/03/19 06:00 [medline]
AID - NEU1116 [pii]
AID - 10.1111/j.1440-1789.2010.01116.x [doi]
PST - ppublish
SO  - Neuropathology. 2010 Dec;30(6):586-96. doi: 10.1111/j.1440-1789.2010.01116.x.