PMID- 20409593
OWN - NLM
STAT- MEDLINE
DCOM- 20101021
LR  - 20211020
IS  - 1873-2534 (Electronic)
IS  - 0165-2427 (Print)
IS  - 0165-2427 (Linking)
VI  - 136
IP  - 3-4
DP  - 2010 Aug 15
TI  - Nitric oxide is elicited and inhibits viral replication in pigs infected with 
      porcine respiratory coronavirus but not porcine reproductive and respiratory 
      syndrome virus.
PG  - 335-9
LID - 10.1016/j.vetimm.2010.03.022 [doi]
AB  - There is little information on the role of nitric oxide (NO) in innate immunity 
      to respiratory coronavirus (CoV) infections. We examined NO levels by Greiss 
      assay in bronchoalveolar lavage (BAL) of pigs infected with either porcine 
      respiratory coronavirus (PRCV) or porcine reproductive and respiratory syndrome 
      virus (PRRSV), a member of Nidovirales, like CoV. The antiviral effects of NO on 
      these two viruses were tested in an in vitro system using a NO donor, 
      S-nitroso-N-acetylpenicillamine (SNAP). We detected a large increase in NO levels 
      in BAL fluids of PRCV-infected pigs, but not in PRRSV-infected pigs. Pulmonary 
      epithelial cell necrosis induced by PRCV coincided with increased NO. Moreover, 
      NO levels in cell culture medium of PRRSV-infected alveolar macrophages (AMs) did 
      not differ from that of mock-infected AMs. Antiviral assays showed that NO 
      significantly inhibited PRCV replication in swine testicular (ST) cells, whereas 
      PRRSV was not susceptible to NO based on the conditions tested. Our study 
      suggests that unlike PRRSV which induces apoptosis in AMs, respiratory CoVs such 
      as PRCV that infect pulmonary epithelial cells and cause cytolysis, induce NO 
      production in the respiratory tract. Thus, NO may play a role in innate immunity 
      to respiratory CoV infections by inhibiting viral replication.
CI  - Copyright 2010 Elsevier B.V. All rights reserved.
FAU - Jung, Kwonil
AU  - Jung K
AD  - Food Animal Health Research Program, Ohio Agricultural Research and Development 
      Center, Department of Veterinary Preventive Medicine, The Ohio State University, 
      1680 Madison Ave., Wooster, OH 44691, USA.
FAU - Gurnani, Ashita
AU  - Gurnani A
FAU - Renukaradhya, Gourapura J
AU  - Renukaradhya GJ
FAU - Saif, Linda J
AU  - Saif LJ
LA  - eng
GR  - R01 AI060739/AI/NIAID NIH HHS/United States
GR  - R01 AI060739-04/AI/NIAID NIH HHS/United States
PT  - Journal Article
PT  - Research Support, N.I.H., Extramural
DEP - 20100401
PL  - Netherlands
TA  - Vet Immunol Immunopathol
JT  - Veterinary immunology and immunopathology
JID - 8002006
RN  - 0 (Nitric Oxide Donors)
RN  - 31C4KY9ESH (Nitric Oxide)
RN  - 79032-48-7 (S-Nitroso-N-Acetylpenicillamine)
SB  - IM
MH  - Animals
MH  - Bronchoalveolar Lavage Fluid/chemistry/virology
MH  - Coronavirus Infections/immunology/*veterinary/virology
MH  - Male
MH  - Nitric Oxide/*immunology
MH  - Nitric Oxide Donors/pharmacology
MH  - Porcine Reproductive and Respiratory Syndrome/*immunology/virology
MH  - Porcine Respiratory Coronavirus/*immunology
MH  - Porcine respiratory and reproductive syndrome virus/*immunology
MH  - Random Allocation
MH  - Respiratory Tract Diseases/immunology/*veterinary/virology
MH  - S-Nitroso-N-Acetylpenicillamine/pharmacology
MH  - Specific Pathogen-Free Organisms
MH  - Statistics, Nonparametric
MH  - Swine
MH  - Virus Replication/immunology
PMC - PMC2902704
MID - NIHMS198440
EDAT- 2010/04/23 06:00
MHDA- 2010/10/22 06:00
PMCR- 2010/04/01
CRDT- 2010/04/23 06:00
PHST- 2009/10/06 00:00 [received]
PHST- 2010/03/23 00:00 [revised]
PHST- 2010/03/25 00:00 [accepted]
PHST- 2010/04/23 06:00 [entrez]
PHST- 2010/04/23 06:00 [pubmed]
PHST- 2010/10/22 06:00 [medline]
PHST- 2010/04/01 00:00 [pmc-release]
AID - S0165-2427(10)00111-X [pii]
AID - 10.1016/j.vetimm.2010.03.022 [doi]
PST - ppublish
SO  - Vet Immunol Immunopathol. 2010 Aug 15;136(3-4):335-9. doi: 
      10.1016/j.vetimm.2010.03.022. Epub 2010 Apr 1.