PMID- 20412420
OWN - NLM
STAT- MEDLINE
DCOM- 20101116
LR  - 20171116
IS  - 1600-0765 (Electronic)
IS  - 0022-3484 (Linking)
VI  - 45
IP  - 4
DP  - 2010 Aug
TI  - Activation of cannabinoid receptor CB2 regulates osteogenic and osteoclastogenic 
      gene expression in human periodontal ligament cells.
PG  - 504-11
LID - 10.1111/j.1600-0765.2009.01265.x [doi]
AB  - BACKGROUND AND OBJECTIVE: Cannabinoid receptor CB2, expressed in osteoblasts and 
      osteoclasts, plays a crucial role in the regulation of bone metabolism. Since 
      periodontal ligament (PDL) cells can differentiate into osteoblasts, this study 
      was undertaken to investigate CB2 expression and the effect of CB2 activation on 
      osteogenic differentiation of PDL cells. MATERIAL AND METHODS: Human PDL (hPDL) 
      cells were obtained from extracted teeth of periodontally healthy subjects. 
      Expression of CB2 was observed in hPDL cells by RT-PCR, Western blotting and 
      immunofluorescence assay. Then hPDL cells were treated with a CB2-specific 
      agonist, HU-308 (10(-7) m), for 12, 24, 48 or 72 h. The mRNA expressions of 
      osteogenic genes, such as runt-related transcription factor 2 (Runx2), bone 
      sialoprotein (BSP), osteopontin (OPN), alkaline phosphatase (ALP), osteocalcin 
      (OC) and collagen type I (COL I), and osteoclastogenic genes, including 
      osteoprotegerin (OPG) and receptor activator of NF-kappaB ligand (RANKL), were 
      examined using quantitative real-time PCR analysis. A mineralization assay was 
      performed in hPDL cells in mineralization conditions with or without HU-308. 
      RESULTS: Expression of CB2 mRNA and protein was detected in hPDL cells. HU-308 
      enhanced the mRNA levels of the above osteogenic genes. Expression of the OPG 
      gene was up-regulated, whereas RANKL gene expression was down-regulated, 
      contributing to the elevated OPG/RANKL ratio. Accelerated mineralization was 
      observed in hPDL cells in mineralization conditions with HU-308. CONCLUSION: Our 
      findings demonstrate that activation of CB2 is able to enhance osteogenic 
      differentiation of hPDL cells and potentially create a favorable osteogenic 
      microenvironment. This implies that CB2 might play an important role in alveolar 
      bone metabolism.
FAU - Qian, H
AU  - Qian H
AD  - Department of Orthodontics, School of Stomatology, Fourth Military Medical 
      University, Xi'an, Shaanxi, China.
FAU - Zhao, Y
AU  - Zhao Y
FAU - Peng, Y
AU  - Peng Y
FAU - Han, C
AU  - Han C
FAU - Li, S
AU  - Li S
FAU - Huo, N
AU  - Huo N
FAU - Ding, Y
AU  - Ding Y
FAU - Duan, Y
AU  - Duan Y
FAU - Xiong, L
AU  - Xiong L
FAU - Sang, H
AU  - Sang H
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20100419
PL  - United States
TA  - J Periodontal Res
JT  - Journal of periodontal research
JID - 0055107
RN  - 0 (Cannabinoids)
RN  - 0 (Collagen Type I)
RN  - 0 (Core Binding Factor Alpha 1 Subunit)
RN  - 0 (Culture Media)
RN  - 0 (IBSP protein, human)
RN  - 0 (Integrin-Binding Sialoprotein)
RN  - 0 (Osteoprotegerin)
RN  - 0 (RANK Ligand)
RN  - 0 (RUNX2 protein, human)
RN  - 0 (Receptor, Cannabinoid, CB2)
RN  - 0 (Sialoglycoproteins)
RN  - 0 (TNFRSF11B protein, human)
RN  - 104982-03-8 (Osteocalcin)
RN  - 106441-73-0 (Osteopontin)
RN  - 8I5L034D55 (HU 308)
RN  - EC 3.1.3.1 (Alkaline Phosphatase)
SB  - IM
MH  - Adolescent
MH  - Alkaline Phosphatase/analysis
MH  - Calcification, Physiologic/physiology
MH  - Cannabinoids/pharmacology
MH  - Cell Differentiation/physiology
MH  - Cells, Cultured
MH  - Child
MH  - Collagen Type I/analysis
MH  - Core Binding Factor Alpha 1 Subunit/analysis
MH  - Culture Media
MH  - Down-Regulation
MH  - Humans
MH  - Integrin-Binding Sialoprotein
MH  - Male
MH  - Osteocalcin/analysis
MH  - Osteoclasts/*physiology
MH  - Osteogenesis/*genetics
MH  - Osteopontin/analysis
MH  - Osteoprotegerin/analysis
MH  - Periodontal Ligament/*cytology
MH  - RANK Ligand/analysis
MH  - Receptor, Cannabinoid, CB2/agonists/analysis/*physiology
MH  - Sialoglycoproteins/analysis
MH  - Time Factors
MH  - Up-Regulation
EDAT- 2010/04/24 06:00
MHDA- 2010/11/17 06:00
CRDT- 2010/04/24 06:00
PHST- 2010/04/24 06:00 [entrez]
PHST- 2010/04/24 06:00 [pubmed]
PHST- 2010/11/17 06:00 [medline]
AID - JRE1265 [pii]
AID - 10.1111/j.1600-0765.2009.01265.x [doi]
PST - ppublish
SO  - J Periodontal Res. 2010 Aug;45(4):504-11. doi: 10.1111/j.1600-0765.2009.01265.x. 
      Epub 2010 Apr 19.