PMID- 20416749 OWN - NLM STAT- PubMed-not-MEDLINE DCOM- 20121002 LR - 20120803 IS - 0309-1740 (Print) IS - 0309-1740 (Linking) VI - 83 IP - 2 DP - 2009 Oct TI - Physicochemical change and protein oxidation in porcine longissimus dorsi as influenced by different freeze-thaw cycles. PG - 239-45 LID - 10.1016/j.meatsci.2009.05.003 [doi] AB - Effects of different freeze-thaw cycles (0, 1, 3 and 5) on physicochemical change and protein oxidation in porcine longissimus dorsi were investigated. When the number of freeze-thaw cycles increased, the thawing losses, cooking loss and b*-value increased (P<0.05), a*-value decreased (P<0.05). The cutting forces of pork increased after one cycle of freeze-thaw (from 28.3N to 40.4N) (P<0.05), but the further increase of freeze-thaw cycles would lead to decrease of cutting force. The decreases in Ca(2+)- and K(+)-ATPase activity and sulfhydryl group (P<0.05) content with concomitant increases in carbonyl content and thiobarbituric acid-reactive substances (TBARS) value (P<0.05) showed that multiple freeze-thaw could cause the porcine protein and fat oxidation, especially for the pork subjected to five freeze-thaw cycles. Gel electrophoresis patterns of porcine muscle showed that multiple freeze-thaw cycles could cause cross-linking of protein in myofibril. Overall, the freeze-thaw process has a detrimental effect on the quality of pork. FAU - Xia, Xiufang AU - Xia X AD - College of Food Science, Northeast Agricultural University, Harbin, Heilongjiang 150030, China; College of Food Science, Heilongjiang August First Land Reclamation University, Daqing, Heilongjiang 163319, China. FAU - Kong, Baohua AU - Kong B FAU - Liu, Qian AU - Liu Q FAU - Liu, Jing AU - Liu J LA - eng PT - Journal Article DEP - 20090510 PL - England TA - Meat Sci JT - Meat science JID - 101160862 EDAT- 2010/04/27 06:00 MHDA- 2010/04/27 06:01 CRDT- 2010/04/27 06:00 PHST- 2008/10/12 00:00 [received] PHST- 2009/04/30 00:00 [revised] PHST- 2009/05/05 00:00 [accepted] PHST- 2010/04/27 06:00 [entrez] PHST- 2010/04/27 06:00 [pubmed] PHST- 2010/04/27 06:01 [medline] AID - S0309-1740(09)00132-6 [pii] AID - 10.1016/j.meatsci.2009.05.003 [doi] PST - ppublish SO - Meat Sci. 2009 Oct;83(2):239-45. doi: 10.1016/j.meatsci.2009.05.003. Epub 2009 May 10.