PMID- 20417307
OWN - NLM
STAT- MEDLINE
DCOM- 20100820
LR  - 20161125
IS  - 0027-5107 (Print)
IS  - 0027-5107 (Linking)
VI  - 699
IP  - 1-2
DP  - 2010 Jun 17
TI  - Frequency of chromosomal aberrations in Prague mothers and their newborns.
PG  - 29-34
LID - 10.1016/j.mrgentox.2010.04.015 [doi]
AB  - The capital city of Prague is one of the most polluted areas of the Czech 
      Republic. The impact of air pollution on the level of chromosomal aberrations was 
      systematically studied: analyses were performed using fluorescence in situ 
      hybridization (FISH) with whole-chromosome painting for chromosomes #1 and #4. In 
      the present study, we analyzed the levels of stable (one-way and two-way 
      translocations) and unstable (acentric fragments) chromosomal aberrations in 42 
      mothers living in Prague and in their newborns. The average age of the mothers 
      was 29 years (range, 20-40 years). Blood samples were collected from October 2007 
      to February 2008. The average levels of carcinogenic polycyclic aromatic 
      hydrocarbons (c-PAHs) and benzo[a]pyrene (B[a]P) in respirable particles (PM2.5), 
      as determined by stationary monitoring, were 21.0+/-12.3 ng/m(3) and 2.9+/-1.8 
      ng/m(3), respectively. We did not observe any effect of either c-PAH or B[a]P 
      exposure on the genomic frequency of translocations (per 100 cells, F(G)/100) in 
      either group due to their similar exposure during the winter months. The mean 
      values of F(G)/100 representing stable aberrations were 0.09+/-0.13 vs 
      0.80+/-0.79 (p<0.001) for newborns vs mothers, indicating a significant increase 
      of F(G)/100 with age. On the other hand, the frequency of unstable aberrations 
      did not differ between the two groups. Our results demonstrate how the patterns 
      of different types of aberration differed between newborns and mothers: we 
      observed 64.3% unstable aberrations and 35.7% stable aberrations in newborns vs 
      19.7% and 80.3% in mothers, respectively. Our results indicate that after birth 
      the frequencies of aberrations are very low and that the aberrations are 
      represented mainly by acentric fragments. The changes observed in mothers show a 
      shift to stable aberrations represented mainly by two-way translocations. The 
      mother's age affected the level of aberrations in newborns: the group of children 
      born to older mothers (31-40 years) had significantly increased F(G)/100 levels.
CI  - Copyright 2010 Elsevier B.V. All rights reserved.
FAU - Rossnerova, A
AU  - Rossnerova A
AD  - Laboratory of Genetic Ecotoxicology, Institute of Experimental Medicine, AS CR, 
      v.v.i., 142 20 Prague 4, Czech Republic.
FAU - Balascak, I
AU  - Balascak I
FAU - Rossner, P Jr
AU  - Rossner P Jr
FAU - Sram, R J
AU  - Sram RJ
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20100422
PL  - Netherlands
TA  - Mutat Res
JT  - Mutation research
JID - 0400763
RN  - 0 (Air Pollutants)
RN  - 0 (Mutagens)
RN  - 0 (Polycyclic Aromatic Hydrocarbons)
SB  - IM
MH  - Adult
MH  - Air Pollutants/*toxicity
MH  - *Chromosome Aberrations
MH  - Chromosome Painting
MH  - Czech Republic
MH  - Female
MH  - Fetal Blood
MH  - Humans
MH  - In Situ Hybridization, Fluorescence
MH  - *Infant, Newborn
MH  - Maternal Age
MH  - Maternal Exposure/*adverse effects
MH  - Mutagens/*toxicity
MH  - Polycyclic Aromatic Hydrocarbons/*toxicity
MH  - Pregnancy
MH  - Translocation, Genetic
EDAT- 2010/04/27 06:00
MHDA- 2010/08/21 06:00
CRDT- 2010/04/27 06:00
PHST- 2010/03/02 00:00 [received]
PHST- 2010/03/26 00:00 [revised]
PHST- 2010/04/10 00:00 [accepted]
PHST- 2010/04/27 06:00 [entrez]
PHST- 2010/04/27 06:00 [pubmed]
PHST- 2010/08/21 06:00 [medline]
AID - S1383-5718(10)00156-7 [pii]
AID - 10.1016/j.mrgentox.2010.04.015 [doi]
PST - ppublish
SO  - Mutat Res. 2010 Jun 17;699(1-2):29-34. doi: 10.1016/j.mrgentox.2010.04.015. Epub 
      2010 Apr 22.