PMID- 20417963
OWN - NLM
STAT- MEDLINE
DCOM- 20100823
LR  - 20191210
IS  - 1878-5905 (Electronic)
IS  - 0142-9612 (Linking)
VI  - 31
IP  - 21
DP  - 2010 Jul
TI  - The use of calcium phosphate nanoparticles encapsulating Toll-like receptor 
      ligands and the antigen hemagglutinin to induce dendritic cell maturation and T 
      cell activation.
PG  - 5627-33
LID - 10.1016/j.biomaterials.2010.03.067 [doi]
AB  - Dendritic cells (DCs) are potent antigen-presenting cells that possess the 
      ability to stimulate naive T cells. Antigen presentation by mature (activated) 
      DCs is a prerequisite for the stimulation of antigen-specific T cells, whereas 
      antigen presentation by immature DCs results in the generation of specific 
      tolerance. Our aim was to develop calcium phosphate nanoparticles which can serve 
      as carriers of immunoactive oligonucleotides into dendritic cells for their 
      activation. We analyzed size, surface charge, and morphology of calcium phosphate 
      nanoparticles loaded with the TLR ligands CpG and poly(I:C) and also with the 
      antigen hemagglutinin (HA) by scanning electron microscopy, dynamic light 
      scattering, Brownian motion analysis and ultracentrifugation. The uptake of 
      fluorescence-labeled nanoparticles into dendritic cells was illustrated by 
      confocal laser scanning microscopy. Immunostimulatory effects of these 
      nanoparticles on DCs were studied, i.e., cytokine production and activation of 
      the cells in terms of upregulation of surface molecules. We show that 
      functionalized calcium phosphate nanoparticles are capable to induce both innate 
      and adaptive immunity by activation of DCs.
CI  - Copyright (c) 2010 Elsevier Ltd. All rights reserved.
FAU - Sokolova, Viktoriya
AU  - Sokolova V
AD  - Institute of Inorganic Chemistry and Center for Nanointegration Duisburg-Essen 
      (CeNIDE), University of Duisburg-Essen, Universitaetsstrasse 5-7, Essen, Germany.
FAU - Knuschke, Torben
AU  - Knuschke T
FAU - Kovtun, Anna
AU  - Kovtun A
FAU - Buer, Jan
AU  - Buer J
FAU - Epple, Matthias
AU  - Epple M
FAU - Westendorf, Astrid Maria
AU  - Westendorf AM
LA  - eng
PT  - Evaluation Study
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20100424
PL  - Netherlands
TA  - Biomaterials
JT  - Biomaterials
JID - 8100316
RN  - 0 (Calcium Phosphates)
RN  - 0 (Hemagglutinins)
RN  - 0 (Ligands)
RN  - 0 (Toll-Like Receptors)
RN  - 0 (Viral Vaccines)
RN  - 97Z1WI3NDX (calcium phosphate)
SB  - IM
MH  - Animals
MH  - Calcium Phosphates/*chemistry
MH  - Dendritic Cells/cytology/*immunology
MH  - Hemagglutinins/*immunology
MH  - Ligands
MH  - Lymphocyte Activation/*immunology
MH  - Materials Testing
MH  - Mice
MH  - Mice, Inbred BALB C
MH  - Nanoparticles/*chemistry
MH  - T-Lymphocytes/*immunology
MH  - Toll-Like Receptors/*immunology
MH  - Viral Vaccines/immunology
EDAT- 2010/04/27 06:00
MHDA- 2010/08/24 06:00
CRDT- 2010/04/27 06:00
PHST- 2010/03/04 00:00 [received]
PHST- 2010/03/25 00:00 [accepted]
PHST- 2010/04/27 06:00 [entrez]
PHST- 2010/04/27 06:00 [pubmed]
PHST- 2010/08/24 06:00 [medline]
AID - S0142-9612(10)00451-5 [pii]
AID - 10.1016/j.biomaterials.2010.03.067 [doi]
PST - ppublish
SO  - Biomaterials. 2010 Jul;31(21):5627-33. doi: 10.1016/j.biomaterials.2010.03.067. 
      Epub 2010 Apr 24.